Study Of The Expression And Significance Of AQP1,AQP2 And AQP3 In Normal Bladder And Bladder Cancer Tissue

Aquaporins(AQPs) is the specific water channel protein ,it resides in animal,plants and microorganism generally. It is the main way that wanter is transported through biomembrane by aquaporins.As yet there are thirteen isforms in mammal cloned by scientist(AQP0~AQP12),their genetic structure,gene expression and regulation,chromosomal localization,protein structure,tussue deposition and physicalogical function have been deeply reached in last decade.Many years study have indicated that the tumor cell has changed a series of enzymetic active and expression for the requirement of the tumor cell proliferation,cleavage,invasion and metastatic, the synthesis of tumor cell elementary structure for instance protein,lipide and nucleic acid was strengthened,even the synthesis of the ribbonucleotide was strengthened also.The protein expression lineage or the protein active was changed.The invasion to interstitial substance and through blood vessel(lympgatic vessel) need chang the volume and shape of the tumor cell. The vital movement of tumor cell need water microenvironment and participation.The tumor cell need more water than normal cell. The elementary biology characteristic of malignant tumor is the infinite proliferation and disdifferentiation of malignant tumor.The tumor cell need more water than normal cell for their proliferation,cleavage,invasion and metastatic.Majority tumor tissue have high tissue space liquid pressure and capillary osmosis.Now a great quantity research have confirmed that AQP high expressed in many kinds tumor tissue,cell line and capillary endothelial cell. So many researchers think that AQPs has relation with the high vascular permeability in tumor tissue. Utilizing the AQPs suppressive agent could significantly degrade tumor metastasize. This illustrate that AQPs can promote tumor metastasis.New viewpoint considered AQPs possess carcinogenesis characteristics. The cDNA of AQPs ectopic expression coule cause many phenoltypic change including cell proliferation et al.The bladder tumor is exogenous noumenon tumor, tumor tissue grew fast, the proliferation and metastasis need a lot of water to metabolism, AQPs participate this process. But now there are a few reports about this, my experiments detect AQPs in normal bladder tissue and bladder tumor tissue by S-P immunohistochemistry,RT-PCR and western blot methods,and suppress the tumor cell proliferation through inhibiting AQPs in the next experiment. Offering a new ways to cure bladder tumor.Method Detect the expression of AQP1,AQP2 and AQP3 in fifty normal bladder tissues by S-P immunohistochemical method and RT-PCR method. Use cystitis glandularis as control group.Next trial using S-P immunohistochemical method,RT-PCR method and Western blot method detect AQP1,AQP2 and AQP3 in bladder tissue. Use normal bladder tissue as control group. Mensurate gray scale of S-P dyeing result, and calculate the immunohistochemical positive exponent through the formula: immunohistochemical positive exponent = exponent area/detecting tissue area×positive gray scale. Use its average scale as positive cell expression rate. The last trial we use the antibody of AQP1,AQP2 and AQP3 to inhibit bladder cancer T24 cell, and use MTT method calculate cell inhibition rate.Results AQP1 expresses in mucous membrane and capillary endothe -liocyte of urinary bladder,AQP2 and AQP3 express in cellular membrane and periplast. The positive exponent of AQP1,AQP2 and AQP3 in bladder tumor was higher than in normal bladder tissue (P﹤0.01). The inhibiting rate of ratio 1:1,1:10 of AQP2 antibody is 23.47% and 16.89% to bladder cancer T24 cell. The inhibiting rate of ratio 1:1,1:10 of AQP3 antibody is 35.63% and 33.51 to bladder cancer T24 cell. The AQP1 antibody had no effect on the bladder cancer T24 cell because AQP1 didn't express in T24 cell.Conclusions AQP1 expresses in mucous membrane and capillary endotheliocyte of urinary bladder,AQP2 and AQP3 express in cellular membrane and periplast; the expression of AQP1,AQP2 and AQP3 in bladder tumor was higher than in normal bladder tissue (P﹤0.01); inhibiting AQP2 and AQP3 could reduce bladder cancer T24 cell proliferation.