Expression And Significance Of Aquaporins In Bladder Urothelial Carcinoma

Purpose:To detect the expression and distribution and investigate it significance of AQP1,AQP2 and AQP3 in normal bladder tissues and bladder urothelial carcinoma tissues. Methods:The expressions and distribution of AQP1,AQP2 and AQP3 were detected by S-P immunohistochemistry and western-blot in the bladder urothelial carcinoma tissues of 25 cases and normal bladder tissues of 25 cases. ResuIts:IHC:The AQP1 was sporadic expressed in the vascular endothelial cells of normal bladder tissues. The majority expression of AQP1 could be detected in the vascular endothelial cells and tumor cells. Sporadic expression of AQP2 could be detected in intercellular substance in both bladder urothelial carcinoma tissues and normal bladder tissues. AQP3 expression was found mainly in the cellular membrane and periplasm of the mucous membrane in both bladder urothelial carcinoma tissues and normal bladder tissues. The expression of AQP1 and AQP3 in bladder urothelial carcinoma tissues was significantly higher than that in normal bladder tissues. The expressions of AQP2 in both were no significant difference. Western-blot:The expression of AQP1 and AQP3 were higher in bladder urothelial carcinoma tissues than in normal bladder tissues. The expressions of AQP2 in both were no significant difference. Conclusion:The expression of AQP1 and AQP3 were higher in bladder urothelial carcinoma tissues and lower in normal bladder tissues. It suggested that AQP1 and AQP3 might relate with the formation and development of bladder urothelial carcinoma and the mechanism need to be further studied.