Treatment Of Ischemic CNS Injury In Mice With Mesenchymal Stem Cells From Murine Fetal Liver

Objectives:To study whether or not murine fetal liver mesenchymal stem cells (flMSCs) could undergo neuroectordermal differentiation with induction of combined neural growth factors; to investigate whether or not murine flMSCs is help to repair CNS injury and recover the neural function in ischemic isogenic mouse; to explore whether or not the signals provided by ischemic CNS microenviroment could induce murine flMSCs to different into cells with neural phenotype; to investigate the migration mechanism of murine flMSCs in ischemic injured CNS.Methods:1. Establishment of an inbreeding murine model of middle cerebral artery occlusion and reperfusionTwo strains of mice (C57BL/6J and BALB/c) were adopted to build model of middle cerebral artery occlusion using thread insertion methods. Model was estimated by neurological deficit evaluation, TTC stain, HE stain and nissl stain at 24 hours after operation. Immunohistochemistry was used to evaluate the damage and regeneration in injured CNS.2. Neural growth factors induce murine flMSCs undergoing neuroectordermal differentiation in vitroflMSCs from 13.5-day-old BALB/c mouse fetuses were isolated by adhering to plastic and passaged using modified method. Cell cycle and phenotype were analyzed by FACSan flow cytometry. Differentiation capability was identified by inducing flMSCs to differentiate into adipose and osteoid tissues. flMSCs were induced with 10 ng/ml b-FGF (basic fibroblast growth factor) for 4 days, 10 ng/ml FGF-8...