Effect Of Different Inducing Conditions In Vitro On Rat Fetal Liver Stem Cells Differentiation

ObjectiveTo explore and compare the effect of different inducing conditions in vitro on differentiation of rat fetal liver stem cells.MethodFetal liver stem cells were isolated on embryonal day (ED) 14 from pregnant F334 rats. The cells were cultured in different mediums containing 10 ng/mL HGF, 20 ng/mL HGF, 40 ng/mL HGF, 0.5% dimethyl sulfoxide (DMSO), 1% DMSO, 2% DMSO, 10 ng / mL HGF +0.5% DMSO, 20 ng / mL HGF +1% DMSO, 40 ng / mL HGF +2% DMSO and the control medium respectively for 15 days. On day 15, the mRNA expression levels of albumin, G-6P, keratin 8 and keratin 18 within cells cultured in different mediums were measured by real-time quantitative PCR. The concentration of AFP and albumin within cells cultured in different mediums were measured by ELISA test. ResultsOn the day 15, the concentration of AFP protein in each experimental group were decreased significantly than the control group (P<0.01), while the concentration of ALB protein were increased significantly(P<0.01); the mRNA expression levels of ALB, G-6P, CK-8 and CK-18 were significantly higher than the control group. 20 ng/mL HGF+1% DMSO was the best inducing condition in vitro on differentiation of rat fetal liver stem cells(P<0.05).ConclusionHGF and DMSO both have concentration- depend effect of inducing the differentiation of fetal liver stem cells in vitro.Furthermore, HGF and DMSO can play a synergistic effect on the differentiation of rat fetal liver stem cell.