Comparing Study On Proteins Expressed During Morphological Development Of C57BL/6 Mouse Inner Ear

Purposes In order to make a good understand of the general principle inthe morphological development of C57BL/6 mouse inner ear and of theexpressions of proteins which were associated with the inner eardevelopment.Methods Take C57BL/6 mouse as experimental animals. The inner earspecimens From E8 to Da of C57BL/6 mouse were made into cryostat sections.Using HE staining, 3D reconstruction technology, SEM and immunohistochemi-strical methods to deal with the frozen sections.Resultsâ‘ Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;â‘¡The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti's tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.â‘¢Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.â‘£Calbindin was notexpressed in spiral ganglion neurons after E16, but expressed continuouslyin scarpa ganglion neurons. However NCS1 can be found expressed in bothof them.⑤Calbindin could be labeled in hair cells between E19 and D22,but no expression was found in adult. And its expression first appearedand last disappeared in inner hair cells: The expression of Myosinâ…¦acould be found clearly between D1 and D7 and after that fading quicklyespecially in outer hair cells.â‘¥many neurons special proteins andneurotrophin factor were expressed in the stria vascularis includingGAP-43, Peripherin, Neurofilament, BDNF, NT3 and TUJ-1 during thedevelopment of the C57BL/6 mouse inner ear.⑦the neuron stem cell marker, Isletl was expressed in the nuclear of the most spiral ganglion neuronsfrom E16 to D7. After 07, the expression was fading;Conclusionsâ‘ The mature time of the hearing function associatedhistological structures suggested that the hearing setout of the C57BL/6mouse should between Dl2 and D14.â‘¡The vessel-like structure beneath theCorti's tunnel during the inner ear development was not spiral vessel(SV).Until now, we can not ascertain the function of this structure andit maybe has some relationship with the development of the corti' s organ.â‘¢scarpa ganglion neurons were different from spiral ganglion neuronsin the biological activity involved in the Ca²⁺.â‘£The time limitedexpression model of Calbindin in hair cells show us its ability to workas the specific regeneration hair cells marker.⑤ruJ-1 were expressedin both afferent and efferent nerve fibers and maybe the afferent nervefibers innervated out hair cells developed further than efferent ones.The Periphein was stained in afferent never fibers and neurofilament200KDin efferent nerve fibers.â‘¥the neuronal original tissue in the striavascularis maybe melanin cells.⑦The spiral ganglion neurons still hadthe characteristics of neuron stem cells before D7 in C57BL/6 mouse innerear.⑧the difference expression of TUJ-1 in the hair cells between avianand C57BL/6 mouse suggested that in normal conditions regenerable andunregenreable hair cells could be distinguished by ruJ-1 staining.