| Objective: To examine the effect of combination of fibronectin precoat and gene transfection by hepatocyte growth factor (HGF) on cell-matrix interaction. Methods: (1) Construction of recombinant adenovirus coding for HGF gene; (2) Isolation and culture of BMSCS; Expression and secretion of HGF in BMSCs after gene transfection; (3) Study on cell-matrix interaction after precoating Fn on decellularized scaffold combined with transfecting HGF gene into BMSCS. Results: BMSCS expressed and secreted HGF efficiently two days after they were transfected. HGF stimulated adhesion of BMSCs to fibronectin in a time-dependent manner with a range of 8-128ng/ml. Histological observation demonstrated a course of BMSCs growth on decellularized valve constructs. A handful of cells, a loose cellular layer, a confluent monolayer coverage, a two-layer structure and a three-layer structure were observed at week 2, 3, 4, 6 and 8, respectively. Immunohistochemical staining revealed cellular reconstitution of endothelial cells and myofibroblasts at week 8. Endothelial cell retention remained high under exposure to high flow and pressure conditions in a bioreactor. Conclusion: The combination of fibronectin and HGF could enhance cell adhesion, re-endothelialization and reconstitution in developing tissue engineering heart valves.
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