Parathyroid Hormone Induced Human Renal Tubular Epithelial Cells CTGF Expression And Regulation Of Genistein

Objective The purpose of our study was to investigate the effect of PTH on the expression of CTGF in human renal proximal tubular epithelial cell line HK-2, determine the signaling pathway which activated and investigate the effect of Genistein on the expression of connective tissue growth factor in HK-2 stimulated by PTH and further the mechanism. Method The expression of CTGF, the machnism by which PTH or Gen acted in HK-2 cells and phenotypic transformation of HK-2 cells was measured by real time-polymerase chain reaction (RT-PCR), western blot, EMSA and light microscopy. Results 1 .PTH promoted CTGFmRNA and protein expression in HK-2 cells with the maximal response of PTH at a concentration of 10"10M and the best stimulating time at 48 hour. 2. PTH increased CTGF expression through EGFR ,MAPK and NF-kB signaling pathway. PTH stimulated ERK phosphorylation depending on three signal transduction pathways: PKA, PKC and PI-3K. 3. Gen inhibited CTGF expression which PTH induced by bloking MAPK and NF-kB signaling pathway. Conclusion l.PTH induced CTGF expression increased in HK-2 cells and transdifferentiation of HK-2 cells. 2. PTH acted through EGFR ,MAPK and NF-kB signaling pathway.3.Gen inhibited the effect of PTH.