Protective Effect Of Ischemic Preconditioning On Reperfusion Injury Of Rat Pancreas

Objectives To establish a convenient model of pancreas ischemia reperfusion inury (IRI) in rats for investigating its negative impact on the function of graft. On the basis of this model, we examined the protection of ischemia preconditioning on the pancreas of rat which have suffered from the ischemia reperfusion. At last, the possible relation between the performance of ischemic preconditioning and overexpress of HO-1 mRNA was been analyzed.Methods Wistar rats were randomly assigned to three groups: (1) sham operated animals without dissection and flushing of the pancreatic tail-segment (Sham, n=20); (2) control animals with dissection of the pancreatic tail-segment, 30 seconds of ischemia by bcloking the abdominal aorta and flushing (Control, n=20); (3) Group IR animals experienced with dissection of the pancreatic tail-segment, flushing, 120 mins of ischemia and reperfusion (IR, n =20 ); (4) animals suffered from a ischemic preconditioning (IPC) performance before the whole process (IPC+IR, n=20 ). IPC was induced by interruption of the arterial blood flow for 3 min followed by 3 min of reperfusion and 6 min followed by 6 min of reperfusion. All groups of animals were sacrificed and were exsanguinated at 0, 2, 4, 6 hours after the reperfusion. Pancreas samples were collected for H&E staining, immunohistchemistry staining and real time PCR analysis.Results fistly, On account of its typical IRI character judged by the change of histopathology and enzymology, the sudy one shows that the The rat pancreas IRI model flushed via celiac axis is a ideal and clinically relevant animal model. Secondly, ischemic preconditioning performed on this kind of model, on the four spots of 0, 2, 4, 6 hours after reperfusion, the extent of Group IPC+IR animals' pancreas histological injury was milder than those of Group IR. The wet-dry ratio was decreas in Group IPC+IR compared with Group IR animals (P < 0.05). on the spot of 2 and 4 hours after reperfusion, the apoptosis index is lower than those of Group IR (P < 0.05). The values of amylase and lipase in Group IPC+IR degrade markly than that of Group IR (P < 0.05). Examined from the results (△Ct mean±SEM) of RT-PCR, the expression of HO-1mRNA in the animals of Group IPC+IR were significantly higher than those of Group IR animals.Condusion The rat kidney IRI model shows a typical IRI. It's a ideal and clinically relevant animal model for the study of pancreas IRI after transplantation, ischemic preconditioning can protect pancreas from ischemia reperfusion injury. IPC can reduce the pancreas anicar cell apoptosis in the early process of IR. IPC could have some important possible relation to the expression of heme oxygenase-1.