Research Of Anticonvulsant And Neuroprotective Effects Of The Tianma Extract (Gastrodin)

Partâ… The experimental observation on the anticonvulsant effect and the effection on epileptic mice behavior of the gastrodinObjective This study compared the anticonvulsant effect by different doses of gastrodin and VPA on PTZ model mice and observed the effects of gastrodin on learning and memory abilities of the epileptic mice by water maze, Evaluating the medicinal value of the gastrodin in the treatment of epilepsy.Methods 50 Kunming SPF healthy adult male mice were divided into five groups : control group, PTZ group, the VPA group, Gs group and Gb group. After training in the water maze, we recorded number of animals mistakes trip and escaping latency before VPA and gastrodin treatment, after treatment, PTZ-induced seizures; and recorded the attack seizure latency and duration, for statistical analysis.Results Significantly prolonged latency was showed in VPA group after drug treatment (p <0.05); the trip latency and the number of errors After induced epilepsy in PTZ group and VPA group was significant difference as compared with the other groups. no significant difference in gastrodin group compared with the control group. After induced epilepsy VPA group compared to PTZ group, Gs group and Gb group, seizure latency were significantly prolonged and convulsion duration shortened significantly (P <0.05); Gs group and Gb group convulsions duration shortened significantly compared to PTZ group (P <0.05). Convulsion intensity group (P=0.796) was no significant difference in each groups.Conclusion Gastrodin can shorten the duration of seizures to certain extent. But the anticonvulsant effect was less than VPA. VPA significantly reduced learning and memory abilities in mice; Gastrodin can inhibit seizures caused decline in learning and memory abilities, with a certain degree of neuroprotective effect.Part two The research on the protective effects on brain for pentylenetetrazol model ratsObjective Application of gastrodin to treat PTZ-induced epileptic rats, and to observe histological changes , apoptosis-related gene expression of caspase-3, GFAP expression and mmp-9 in the brain hippocampal neurons, glial cells and endothelial cells, and to explore the protective effects and possible mechanism of gastrodin on neurons, astrocytes and endothelial cells.Methods 102 SPF healthy adult male Wistar rats were randomly divided into Control group, PTZ group, the VPA group, Gs group and Gb group. PTZ-induced seizures were given VPA and gastrodin treatment, and to death the animal for materials at 12 hours, 48 hours, 5 days and 7 days. By HE staining, TUNEL, caspase-3, MMP-9 and GFAP immunohistochemistry, to observe hippocampal CA1, CA3 and dentate gyrus histological changes and to analysis each time point IOD in each group.Results HE staining in PTZ group after seizures showed notable neuronal degeneration and necrosis in the hippocampus regions of CA1 and CA3, and less serious in region of hilus of the dentate gyrus. the more serious loss of neurons the more time extension. VPA-treated group and the gastrodin-treated group showed lesser extent damage. Immunohistochemistry results in PTZ group showed high expression in TUNEL and caspase-3 staining in the regions of CA1 , CA3, hilus of the dentate gyrus and vascular. 5day reached its peak. IOD had a significant difference compared to VPA group, Gs group and Gb group. GFAP and MMP-9 in these regions also have high expression in PTZ group, more visible on 5d-7d. and IOD a significant difference compared to the VPA group, Gs group and Gb group.Conclusion PTZ-induced epilepsy model can lead to a clear-induced damage on hippocampus neurons, astrocytes and brain vascular. And the large or small dose of gastrodin and VPA can reduce the apoptosis and necrosis of neurons and endothelial cell in the hippocampus. The effects of gastrodin and VPA are similar. Gastrodin protect brain tissue from injury caused by seizure on neurons, glial cells and endothelial various levels. Its neuroprotective mechanism might involve inhibition of the pro-apoptotic gene expression of caspase-3; inhibited GFAP over expression; inhibition of MMP-9 expression induced pro-inflammatory response.