Research On The Regulation Of TGF-β1/Smad3 Signal Transduction Pathway On Derma Fibroblasts During The Wound Healing Process

During the wound healing process of severe burn trauma, skin fibroblasts play a major role. TGF-β1/Smads signal transduction pathway has close relationship with wound healing. To illustrate the precise regulation of this signal transduction pathway on skin fibroblasts duing the wound healing process, Smad3 knockout mice and specific signal transduction pathway kinase inhibitors were used in animal models and cell experiments. Research confirmed that TGF-β1/Smad3 signal transduction pathways involved in the wound healing process and after the trauma showed a brief activation; Smad3 negatively regulated the effect of TGF-β1 on promoting wound healing. Smad3 inhibited the positive feedback of TGF-β1 and contributed to the proliferation of fibroblasts, also mediated negative regulation on MMP2/9 and positive regulation on TIMP-1. During the process of fibroblasts transdifferentiation into myofibroblasts induced by TGF-β1, Smad3 pathway mediated negative regulation, while p38/MAPK and JNK/MAPK pathway mediated positive regulation.