Study On The Effects And Influencing Factors Of BG Composite Bacteria In Aerobic Fermentation Of The Organic Mixed Waste

The research focuses on the methods of laboratory and mechanized productiontest which were used to study the BG composite bacteria conditions and the organicmatter decomposition during the aerobic fermentation process of organic mixed wastein order to solve the drawbacks of conventional treatment of mixed organic waste(forest humus, household waste, kitchen waste, animal manure, crop straw, etc.), theserious soil degradation of agricultural production and the lack of bio-organicfertilizer. Traditional methods and16SrDNA molecular biology methods were used ineach stage to study the fermentation beneficiation microbiology. The research alsostudied the fermentation oxygen content, temperature, humidity, pH value, organicmatter, and the C/N-ratio variation with the days of fermentation. Moreover, westudied the effect of Oxygen content, temperature, humidity, pH value, organic matter,and the C/N-ratio in the bacteria, fungi and actinomycetes and degradation of organicmatter. The aerobic fermentation product of bio-organic fertilizer were detected andtested, as well. The main results were listed as below:1. Humus of forest areas, household waste, kitchen waste, animal manure, andcrop stalks of organic waste were mixed in proportion to start the aerobicfermentation. Constantly separated and purified for dominant microflora, and obtainedbacillus in beneficial microorganisms by fostering, we called its Bacillus Genus (BG)composite bacteria.2. Organic mixed waste such as raw material in the whole experimental processcreate a suitable living environment for beneficial microorganisms, nurturing innature widely distributed bacteria, fungi, and actinomycetes and allowing their growthand reproduction rapidly. During the growth process of bacteria, fungi, andactinomycetes, due to the decomposition of organic matter and heat released, thetemperature of the fermenting material was elevated, and the process promotes therapid degradation of various types of organic matter fermented material. Organicmixed waste aerobic fermentation experiment is the process of strengthening thenature microbial degradation of organic matter, and BG composite bacteria are the main function organism of biological degradation of organic matter in the aerobicfermentation process. Consequently, the high temperature period is an important stageof rapid degradation of organic matter and other substances.3.The changes in the physical and chemical indicators were managed, and after25days of aerobic fermentation, the indicators stabilized, and the organic wastecomposted completely. Nutrients were tested in accordance with the People'sRepublic of China Agricultural Industry Standards (NY884-2004), and they finallyreached the national targets of bio-organic fertilizer and achieved the reduction,harmless, and the resources of the organic mixed waste.4. The study used microbial plate count method in traditional training methods todeterminate changes in the microorganisms during the aerobic fermentation offermented material. The results showed three similar "increases-decrease" trends ofthe total number of bacteria, fungi and actinomycetes. Changes of temperature andorganic matter influence the bacteria, fungi, and actinomycetes and play a screeningrole during species screening. Isolated purified strains were analysised by usingphysiological and biochemical method and16SrDNA of molecular Biology method.DNA sequence comparison results indicated that six samples of total seven sampleswere Bacillus close, which indicating that the thermophilic BG composite bacteria arethe main microbial degradation of organic matter not only in the aerobic fermentationof organic mixed waste, but also one of the dominant flora in cellulose, hemicellulose,and lignin degradation.5. We measured the oxygen concentration, temperature, pH value, humidity,organic matter containing, and the C/N-ratio changes with the number of aerobicfermentation, and we found that the oxygen concentration, temperature, pH, humidity,organic matter containing rates, C/N-ratio are important factors in aerobicfermentation of organic waste. These factors impacted the number, the species, andthe degradation of organic matter by the speed of growth and reproduction of bacteria,fungi, and actinomycetes. Six factors affected interdependent and complementary, andeach factor has its appropriate scope. Whether it is more than appropriate range or lack of will affect the growth and reproduction of microorganisms, every factoraffects the degree of degradation of organic waste.6. In the mechanization of the organic mixed waste aerobic fermentation test, wefollowed the environmental philosophy, which not adding any special bacteria, livingwaste without sorting and without deodorant device. Through strict monitoring andtimely adjustment of six influencing factors, which are the oxygen concentration,temperature, pH value, humidity, the rate of organic matter containing and theC/N-ratio, not only do we greatly improved the efficiency of the organic wastetreatment and disposal, but also we produced high-quality bio-organic fertilizer in linewith national targets.