Electric And Electrochemical Responses Of Adherent Cells: Application Of Microfabrication Technologies

Understanding the cell behaviors such as cell adhesion, migration and proliferation is important for tissue engineering and implantology. This thesis work has been developed to obtain a more clear insight on in-vitro cell culture behaviors by using both micro/nano fabricated substrates and electric/electrochemical methods for continuous and nondestructive monitoring. A variety of patterned substrates, including high density nanoelectrodes and three-dimensional pattern networks, could be obtained for cell culture and electric/electrochemical analyses with or without microfluidic device integration. Cell adhesion, migration and proliferation as well as cellular metabolic activity were monitored by cyclic voltammetry and impedance techniques. In combination with optical methods for the observation of cell morphology and cell densities, electric/electrochemical methods allowed us studying a number of new effects of patterned substrates or electrodes on cell culture behaviors and cellular metabolic activities.