| Perilipin and Adipocyte differentiation-related protein were located on the surface of lipid droplets, which were two members of the PAT family. Because both of them not only participated in the lipid metabolism, but also play a part in 2 type diabetes, adiposity and artery atherosclerosis. Peroxisome proliferator-activated receptor-y (PPARy) is a member of the nuclear hormone receptor superfamily of ligand-dependent transcription factors and regulates the expression of genes involved in the transcriptional control of energy, lipid, and glucose homeostasis.The current research chose Zhejiang White goose and Landes goose liver and fat tissues as the materials. We successfully obtained the coding sequence of goose PLIN gene, which was 1589bp. GenBank assigned them accession numbers GU938447, and cloned the part sequence of ADFP, which was 1304bp. Via bioinformative analyses, we found both the two amino acid sequences had the structural characters of PAT family and these two genes had high identity with with other species, respectively. Thus, we concluded that they were the protein of goose ADFP and PLIN. We inferred they had the function on controlling goose lipid metabolism.The present study took Zhejiang White geese and Landes geese as the materials. We used the real-time PCR method to test the expression quantity of ADFP, PLIN and PPAR y gene in the breeds mentioned above at different nine points after birth, and we also tested the expression quantity of ADFP and PLIN gene in different growth points and had correlation analysis of these relative quantities and traits in two breeds.ADFP expression is more widespread in many tissues, yet PLIN gene and PPAR y mRNA expressed mostly in adipose tissues. The results indicated that there were advantageous tissues for ADFP, PLIN and PPARy gene expressions at each growth point and there were also advantageous growth points for gene expressions in a certain tissue, and the expressions of genes were significantly difference in Zhejiang White goose and Landes goose. In adipose tissues, ADFP values present the high then descend tendency. However, PLIN value show the contray the tendency with the development of goose growth, the change day was the 28d after birth. PPARy expressions in tissues were lower than the two others relatively. We inferred they had the function of controlling goose lipid metabolism with the adipocyte mature.The current study adopted Zhejiang White goose in Zhejiang province. We screened the extrons sequences of ADFP gene and PPARy gene using PCR-SSCP and sequencing method. We got nine and one single nucleotide polymorphisms (SNP) of ADFP and PPARy gene, respectively. The allele G, G, G, C, T, G, C, T and C were the predominant allele in SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and SNP9, respectively. There was complete linkage disequilibrium between SNP3 to SNP9. The results of single locus analysis showed that SNP2 was significantly associated with percentage of leg muscle (P<0.05) and extremely significant genetic effect on breast muscle weight and percentage of breast muscle (P<0.01); SNP3 was significantly associated with breast muscle weight and leg muscle weight (P<0.05), extremely significant genetic effect on percentage of breast muscle(P<0.01), there was no predominant allele at SNP2. However, allele G was the predominant allele at SNP1. And the SNP2 to SNP9 had middle polymorphic informative content (PIC). PPARy SNP was significantly associated with percentage of leg muscle (P<0.05).Generally, the results of marker-trait linkage analyses indicated that the polymorphisms of ADFP and PPARy were significantly associated with carcass traits. We inferred that ADFP and PPARy probably were the major genes affecting carcass traits in goose. |
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