| The liver metabolize of goose was dramatically changed induced by overfeeding, and causing large fats deposited in liver. At present, the molecular mechanism of fatty liver forming is still remaining un-clear.In this study, mRNA differential display technique was applied to isolate the differentially expressed gene between fatty liver and normal liver of Landes and Xupu goose. Ninety primer pairs were used and approximately eight hundred pieces of cDNA segments were analyzed in this study. Eventually six differentially expressed genes were confirmed by using real-time quantitative PCR technique, among which gene 9105 was confirmed as up regulated in both fatty livers. And besides, gene 9105 was predicted by the bio-informatics technique. The result indicated that cDNA fragments of gene 9105 which was obtained though cDNA cloning contained an open reading frame of 1239 bp which encoded a protein of 412 amino acids, and the sequence of this fragment showed homologous with the mRNA transforming growth factor- beta 2 (TGF-β2) of chicken and other species, and the protein of 412 amino acids contained two putative conserved domains of TGF-β-propeptide and TGF-β, and has high homology with its homologues-chicken 99%, Xenopus laevis 96%, Monodelphis domestica 91%, human 90%, pig 90%, cattle 89%, Ovis aries 89%, mouse 88%, rat 87%. And by semi-quantitative RT-PCR technique the result showed gene 9105 was expressed in manifold tissues. The expressed level is the highest in muscular stomach, secondly in liver, spleen and ovary, thirdly in uterus, lung and muscle, the lowest in abdominal fat and kidney.
|
PDF Full Text Request