Studies On The Efficient Preparation Of The Active Compounds From Echinacea Purpurea (L.) Moench,Purple Sweet Potato And Biological Activities Of Echinacea Purpurea

This study has two parts which one deals mainly with chemical, bioactive investigation and HPLC detection method on Echinacea purpurea (L.) Moench and another deals with chemical on Purple sweet potato, the main results are as follows:1,Set up High-speed countercurrent chromatography and preparative HPLC methods to separate cinnamic acids and alkylamides in Echinacea purpurea, fifteen high-purity compounds were got from Echinacea purpurea and thirteen structures were identified by MS, NMR, UV, three compounds such as2-O-isoferuloyltartaric acid,2-O-caffeoyl-3-O-isoferuloyltartaric acid,2,3-di-O-isoferuloyltartaric acid were new compounds.2,Set up a High-speed countercurrent chromatography mothed to separate four compounds from purple sweet potato, the structure of them identified by MS, NMR, UV, the resuls showed two of them were acylated anthocyanins and anther were chlorogenic acid and ischlorogenic acid A.3,The model of inhibition influenza virus neuraminidase enzyme has been used to study the compounds in Echinacea purpurea on the neuraminidase of influenza virus A/PR/8/34(H1N1). The results showed that cinnamic acids have significant inhibitions, cinnamic acid compound3was the strongest and IC50values was5.68±0.19μg/mL. Suggesting that one pathway of antiviral activity of Echinacea purpurea was the inhibition of virus neuraminidase enzyme.4,Sulfonyl rhodamine B staining method has been used to study the compounds of Echinacea purpurea on BEL-7402human hepatoma and MCF-7human breast cancer cell lines. The results showed that cinnamic acids and alkylamide compounds have weak inhibition on tumor cell, while the mixture of alkylamides were strong inhibition compare with purity compound, IC50values were0.05mg/mL and0.03mg/mL respectively.5,The model of antagonism has been used to study the compounds of Echinacea purpurea on chemokine receptor CXCR4and CCR5. The results showed that all the samples only have a weak antagonism, suggesting it was not obviously that Echinacea against HIV in the infection of host cells.6^Study the inhibition of compounds of Echinacea purpure on four kinds of bacteria. The results showed there were no inhibition on bacteria such as Staphylococcus aureus, Escherichia coli, Salmonella and hemolytic streptococcus.7,Chemical method have been used to study antioxidant activities of cinnamic acid derivatives of Echinacea purpure, the results showed that the antioxidant of compounds were as follows:Cichoric acid,2-caffeoyltartaric acid,2-caffeoyl-3-feruloyltartaric acid,2,3-diisferuloyltartaric acid,2-isferuloyltartaric acid; Structure-activity relationship showed:active groups of antioxidantactive was the phenolic hydroxyl on benzene of caffeic acide groups, and the bit phenolic hydroxyl was the strongest activity and adjacent was weak, but the protective effect of DNA damage does not exist this relationship, indicating that the protective effect has different action mechanism.8,Simultaneous analysis7cinnamic acid compounds and5alkylamide compounds in Echinacea purpure by HPLC-PDA with gradient elution on a C18reverse phase column in60minutes without the pretreatment of samples, it was suitable as standard analytical method to detect the active ingredients in Echinacea purpure.