| The Vitreoscilla hemoglobin gene (VHb), trans-zeatin secretion gene (tzs) andthe modified 5-Enolpyruvylshikimate-3-phosphate synthase gene (EPSPS), aslinked expression cassettes, were simultaneously introduced into immatureembryos of the rice (Oryza sativa L.) varieties 'Xiushui-11', 'Qiufeng','Youfeng'and 'Hanfeng'by Agrobacterium tumefaciens. A total of 1153transgenic lines, composed of 4222 plants were obtained through selection forhygromycin B resistance. Genomic PCR, Southern and northern blottinganalyses and other relative tests showed that all transgenes had been integratedinto the rice genome and expressed effectively. Approximately 90.2% of thetransgenic lines harbored all the transgenes. Expression analysis revealed that alltransgenes co-expressed stably in transgenic plants, and the frequency ofco-expression was about 85%. Statistically significant increases were observedin plant height, panicle length, total grains per panicle and filled grains perpanicle in transgenic plant lines compared with the control. Our studydemonstrates a possible way to introduce different transgenes as linkedexpression cassettes within a single vector into the plant genome. Moreover this...
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