Control The Morphology Of The Completion Of Related Genes In Rice

Rice (Oryza sativa L.) is an important crop for the world, and it has been studied extensively for a long time. Recently, with the rapid progress of molecular biology and genomics, the studies on rice floral development have been conducted at the molecular level. Our study mainly focuses on three genes which might determinate rice architecture——one is OsTb1, Oryza sativa Teosinte Branched 1-like gene, and the other two are OsCen1 and OsCen2,Oryza sativa Centroradialis-like genes in rice. Our aim is to understand the process of rice development at the molecular level, and to reveal the mechanism controlling rice tillers and inflorescence architecture. Since the production of rice is related to the development of tillers and spikelets, our research could have direct application to the yield improvement. It has been showed that Tb1 controls the tillers initiation and development in maize (Zea mays L.). With the ORF of Tb1 as a probe, we screened a rice phage genomic library, and obtained several positive clones. The analysis of one clone showed that it had the TCP and R regions that were most conserved among the TCP genes, such as Cyc and Tb1.We also cloned its cDNA by RT-PCR and 3'-RACE and found this gene had no introns. This gene was a new member of TCP gene family in rice, and we named it as OsTb1 after Oryza sativa Teosinte Branched 1.The length of its ORF was 1164 bp, encoding a polypeptide consisting of 388 amino acids. The similarity at the level of amino acids between Tb1 and OsTb1 was more than 70%, and the identity in the TCP region was more than 93%. We detected its expression in rice tillers with PCR-Northern and mRNA in situ hybridization. We also constructed both sense and antisense plasmids of OsTb1 for rice transformation via Agrobacterium tumefaciens. Our lab has cloned two copies of Cen-like gene in rice---OsCen1 and OsCen2, and our preliminary results showed that they were related with rice flower development. We constructed both sense and antisense plasmids of OsCen1 and OsCen2 for rice transformation via Agrobacterium tumefaciens and analysed the phenotype of the rice primary transformants. Comparing with the wild type, the rice primary transformants with the ectopic overexpression of OsCen1 and OsCen2 did have significant changes with its inflorescence: the increase of the...