Studies On The Expression And Function Of The Key Gene FZP Controlling Flower Development In Rice (Oryza Sativa L.)

The studies on molecular genetics of flower development have become a hotspot about molecular biology of plant development since 1990. The unicotyledonous plant rice is an important cereal. For its panicle (inflorescence) contributes to the majority of our harvest, it is very important to know at the molecular level the genes related to the development of the panicle. Frizzy panicle (FZP) is a key gene for spikelet differentiation in rice. Liu huaqing et al (2003) had found a gene which have the same domain with APETALA2 (AP2) gene by using high-resolution mapping on chromosome 7, and took it as the, most possible candidate of FZP. In the same time, Komatsu et al (2003) had found FZP gene on the same position. We connected FZP promoter with report gene (GUS), and connected the maize ubiquitin promoter (Ubi-1) with the promoter of FZP gene, then construct space-and-time expression and overexpression vector of FZP gene, which will be transferred into rice finally, and try to understand the expression pattern of the FZP promoter and the functions of the target gene in rice by analyzing the transgenic plants showing its space-and-time expression and overexpression pattern.At first, the promoter of FZP gene and FZP cDNA which had been amplified from rice genome were transferred into pMD18-T cloning vector for DNA sequencing and double digestion. Then, the digested target DNA segments were inserted into the T-DNA region of vector pCAMBIA1381z and pTCK303 individually, which will then be transferred into A.tumefaciens EHA105 to get the stains for transformation. Mature embryos of japonica Nipponbare initially cultured for 4 days were infected and transferred by these A.tumefaciens with different expressive vector separately. After selection and re-differentiation for...