| Objective:To study index levels of energy metabolism as of filial generation born of the middle cerebral artery occlusion (MCAO) model rats with blood-stasis-due-to-qi-deficiency syndrome (BSDTQDS) as well as normally contemporary SD rats after being syndrome molded and to compare changes in qi-deficiency syndrome grading, blood-stasis syndrome grading and related ethology indexes before/after intervention treatment by a TCM formulation, the Buyanghuanwu Decoction. And next to study index levels of hemorheology, thrombocyte membrane glycoprotein etc. as of filial generation born of the MCAO-model rats with blood-stasis-due-to-qi-deficiency syndrome(BSDTQDS) after being molded as previously stated in Part 1, to observe related pathological transformation as of arteria carotis if any as well as to compare with normally contemporary rats to study whether there is any tendency to result into physiological/pathological status of pre-ischemic stroke for filial generation of the former.Method:Part 1:Filial generation rats were born through mating between male/female SD rats after BSDTQD syndrome molding and MCAO apoplexy molding and, such subjects were made syndrome molding once again to produce 10 filial generation of syndrome experimental group(SEG) and compared with 10 normally contemporary SD rats by the same syndrome molding in syndrome controlled group(SCG) and other 10 normally contemporary SD rats without any intervention involved in blank controlled group(BCG) to study index change levels of their energy metabolism like lactic acid, creatine kinase and LDH and to compare changes in qi-deficiency syndrome grading, blood-stasis syndrome grading and related ethology indexes like open-field test, net-climbing test before/after intervention treatment by Buyanghuanwu Decoction.Part 2:Content levels of hemorheology, content level of thrombocyte membrane glycoprotein as GPâ…¡b/â…¢a and CD62P plus physiological/pathological status of vascular endothelium at initial section of the arteria carotis interna for filial generation of the MCAO-model rat with BSDTQDS previously stated was surveyed through syndrome molding of 10 filial generation subjects in syndrome experimental group(SEG) and comparison with 10 normally contemporary SD rats in syndrome controlled group(SCG) with the same syndrome molding and other 10 normally contemporary SD subjects in blank controlled group(BCG) without any intervention involved.Result:Part 1:Having being molded, related examinations were done as followed.1st, energy metabolism index evaluation, level of each energy metabolism index of the SEG was higher than the two other controlled groups with statistically significant difference. As for SCG and BCG, level of each index as of the former was also higher over the latter. 2nd, qi-deficiency syndrome grading, blood-stasis syndrome grading and assessment of related ethology indexes, before treatment (to speak exactly, this experiment), each index assessment of SEG showed statistically significant difference in comparison with others, and both qi-deficiency syndrome and blood-stasis syndrome of SEG were more serious than controlled groups. Meanwhile, qi-deficiency/blood-stasis syndrome of SCG was more serious than the other and, there was statistically significant difference in each index between groups. After treatment, each assessment was improved as for both SEG and SCG, and assessment of SEG still manifested statistically significant difference with the others. Despite the fact that there were statistical differences in index assessment of blood-stasis syndrome and net-climbing test between controlled groups, results of qi-deficiency syndrome index, open-field test as well as weight index showed no statistically significant difference between them.Part 2:Having syndrome molding, there were statistically significant differences in hemorheology content level and content level of thrombocyte membrane glycoprotein as GPâ…¡b/â…¢a and CD62P as of filial generation in SEG in comparison with the other ones with hemorheology of these subjects showing the state of obviously high blood viscosity and content level of thrombocyte membrane glycoprotein as GPâ…¡b/â…¢a and CD62P significantly higher over their counterparts. In contrast to subjects in BCG, there was also statistically significant difference in the change of hemorheology for the ones in SCG with blood showing mildly high viscosity, while there wasn't statistically significant difference in content level of thrombocyte membrane glycoprotein as GPâ…¡b/â…¢a and CD62P between such two groups. As for pathology examination on subjects in the three groups, HE coloration at initial section of the arteria carotis interna demonstrated apparent edema/damage in SEG, which proved clear change compared with its counterparts and, endothelial cells were in edema state as of SCG, which was very slight and not obvious as SEG. Furthermore, there was not any abnormality of the pathological section of the endothelium in BCG.Conclusion:1. Both index assessment and treatment counterevidence suggest that, filial generation born by MCAO rats and normally contemporary rats can be induced to fall into evident BSDTQDS under intervention of syndrome molding factors. What's more, filial generation of the MCAO-model rats with BSDTQDS could be likely led by molding of BSDTQDS to more serious BSDTQDS manifestations than normally contemporary rats.2. Intervention on syndrome molding factors helps both filial generation of the MCAO-model rats with blood-stasis-due-to-qi-deficiency syndrome and normally contemporary rats fall into myxemia and produce pathological changes like edema, damage, etc. in endothelial cells of arteria carotis interna as for rats, however, increase of thrombocyte adherence function and thrombosis tendency is only found in SEG rather than SCG. Thus comparing to normally contemporary rats, filial generation of the MCAO-model rats with BSDTQDS could be likely induced by molding of BSDTQDS to lead to more obvious myxemia, pathological changes like edema, damage, etc. and the tendency of thrombosis than normally contemporary rats.
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