| Objective:The subject of clinical observation, mainly through the blood to heat syndrome of psoriasis vulgaris by Jia Wei Liang Xue Xiao Feng San PASI score before and after treatment, the symptom score, changes in the degree of itching, preliminary evaluation of this side effect, while its safety evaluation; through the guinea pig animal model of psoriasis psoriasis side effect of this mechanism and explore its role in psoriasis vulgaris part of the mechanisms.Methods:Clinical study:60 cases will be carried out phase of blood-heat syndrome in patients with psoriasis vulgaris were randomly divided into flavored Jia Wei Liang Xue Xiao Feng San group of 30 cases and compound Indigo capsule group of 30 patients were given flavored cooling blood and compound Indigo Xiaofengsan capsule treatment,4 weeks for a course of treatment, continuous observation of two courses, and after treatment to determine efficacy, while during the period of the trial in the treatment of drug safety evaluation. Experimental Study:Using generally accepted 5% propranolol-induced animal models of psoriasis as an object of study, the main observation of experimental drugs on the model of lesions, TNF-a, PCNA role and impact of research. The organizational structure of the use ofradioimmunoassay, immunohistochemistry and other advanced experimental techniques and research tools, from the cellular ultrastructure, cytokines, proteins and other aspects of the organization flavored Jia Wei Liang Xue Xiao Feng San period of treatment for abnormal blood heat syndrome the mechanisms of psoriasis are discussed.Results:The clinical observation showed that:Jia Wei Liang Xue Xiao Feng San treated 30 cases of blood-heat syndrome type to view psoriasis, two markedly after treatment in 22 cases, effective in 7 cases, ineffective in 1 case, the total effective rate was 73.33% and the total effective rate of 96.67%. Slightly better than its efficacy through statistical compound Indigo capsule group (Z=-2.001, P=0.045<0.05). This test Modified cooling blood in the clinical observation of Xiaofengsan string not found any adverse reactions. Experimental results show that:induced animal model in guinea pigs after modeling were compared with the control group showed typical hyperkeratosis Bank of psoriasis, and other pathological manifestations of parakeratosis; diseased tissue tumor necrosis factor-α(TNF-α), proliferating cell nuclear antigen (PCNA) showed high expression.After Modified Liangxue Xiaofengsan treatment, the ear level of the organization keratosis, inflammation and other pathological conditions have markedly improved, the medicine dose group compared with the model group were statistically significant degree of inhibition keratosis; high dose of Chinese medicine compared with the model group has obvious anti-inflammatory; short flavored Liangxue Xiaofengsan inhibit psoriasis-like lesions induced by the pathological changes.At the same time by the Chinese group and positive control animals treated ear tissue content of TNF-αcompared with the model group decreased, and MTX control group and model group was statistically significant P<0.05, high dose group and model group statistically significant P<0.05, MTX control group and low dose group was statistically significant P<0.05. But the medicine of high, medium and low dose chart shows the dose from among a certain relationship, but not significantly.The Chinese group (high, medium, low) and positive control animals treated ear tissue expression of PCNA, icons can be seen from the group had a lower Chinese high, medium and low doses of dose-effect between the chart shows there though certain relations, but not significantly.Conclusion:The clinical trials Tip:Modified cooling blood Xiaofengsan period of treatment for psoriasis of blood-heat syndrome has good efficacy and safety; animal research suggests that:Modified cooling blood Xiaofengsan better anti-inflammatory, inhibit keratinocyte The role of keratinization forming cells, through the cooling blood test revealed Xiaofengsan Modified by inhibiting TNF-αto regulate the apoptosis of keratinocytes to achieve anti-inflammatory, inhibit keratinocyte keratosis role.
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