Experiment At Study Self-made Freeze-dried Bilayered Fibrin-binding Amniotic Membrane To Inhibit Scarring After Glaucomatous Filtering Surgery

Objective:To investigate the antifibrotic effect of the freeze-dried bilayered fibrin-binding amniotic membrane on glaucoma surgery in rabbit model.Method:Twenty-four Japanese white rabbits were randomlyinvaded into three groups: the experimental group (ocular trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane transplantation), the control group (ocular trabeculectomy in combination with natural bilayered fibrin-binding amniotic membrane) and the blank group (single trabeculectomy). Clinical observation, HE staining, massion staining, Real-time per and immunohistochemistry for a-SMA were performed on days 7, 14,21 and 30 following surgery.Results:Statistical differences were noted in conjunctival congestion and intraocular pressure among groups on days 7,14,21 and 30 following surgery. Histology and Real-time PCR further demonstrated that trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane yielded well wound healing and no scar formation and was beneficial for long term effect. Objective:To investigate the antifibrotic effect of the freeze-dried bilayered fibrin-binding amniotic membrane as a drug delivery system on glaucoma surgery in rabbit model. The aim of this study was to prepare a novel local delivery system for the sustained and controllable release of 5-Fu.Method:Twenty-four Japanese white rabbits were randomized into three groups:the experimental group (ocular trabeculectomy in combination with 5-Fu loaded freeze-dried bilayered fibrin-binding amniotic membrane transplantation), the control group (ocular trabeculectomy in combination with 5-fu) and the blank group (single trabeculectomy). HE staining, massion staining and immunohistochemistry for a-SMA were performed on days 7,14,21 and 30 following surgery.Results:Statistical differences were noted in intraocular pressure among groups on days 7,14,21 and 30 following surgery. Histology further demonstrated that trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane yielded well wound healing and no scar formation and was beneficial for long term effect. HPLC showed a good slow-release effect with freeze-dried bilayered fibrin-binding amniotic membrane。...