| ObjectiveThis study was designed to evaluate the roles of matrix metalloproteinase-2(MMP-2) and tissue inhibitors of Metalloproteinase-2(TIMP-2)in subconjunctival tissue wounding healing after glaucoma filtering surgery. Experiments were performed in a rat model.Methods Glaucoma filtering surgery was performed on60Sprague-Dawley rats by introducing a anterior chamber silicone cannula. Respectively at days1,3,5,7,14and28after operation they were killed in batch. Tissue was harvested from the conjunctival and subconjunctival tissue. One half of the bleb tissues were made pathological section, and then were H-E stained and observed under light microscope. The others of the tissues were detected by semi-quantitative western blot analysis.Results1. Following the cannula GFS, well-elevated filtering blebs formed and gradually failed over the course of7to17days, with a mean time to complete failure was12days.2. Histological examination showed that inflammatory cells, fibroblasts, vasodilatation and collagen occurred in the progress of scarring formation.3. Western blot results:MMP-2was down-regulated1day after surgery, up-regulated3days after surgery and observed with a peak at7days after surgery, and then it persisted until28 days. The difference was statistically significant(p<0.01).TIMP-2was up-regulated1day after surgery and observed with a peak at5days after surgery, and then it persisted until28days. The difference was statistically significant(p<0.01).Conclusion In the progress of rat subconjunctival tissue wound healing, the expressions of MMP-2and TIMP-2were changed at protein levels. It suggests that inhibition of TIMP-2to MMP-2, that action may help develop the new anti-scarring drugs. |
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