Effect Of Ulinastatin Donor-pretreatment On Liver Graft During Cold Preservation In Rats

Background:To determine whether donor-pretreatment with Ulinastatin can attenuate liver cold preservation injury, and to explore the mechanism of Ulinastatin affecting donor liver graft.Methods:144 Wistar rats were divided into Ulinastatin treatment group (T group) and control group (C group), pretreated with Ulinastatin 50 OOOU/kg or 0.9%NS injection via peritoneum prior to the anesthetization. After the abdominal cavity was opened and perfused with cold Ringer's lactate solution, the liver was harvested. The harvest liver was preserved in cold Ringer's lactate solution for Oh,2h, 6h,18h,24h, at which time the liver tissue was sampled for determination of dry weight to wet weight, Na+-K+-ATPase, Ca2+-ATPase activity, lactic acid dehydrogenase activity, lactic acid and malondialdehyde levels. Light microscopy and electron microscopy were used to detect liver morphocological observation. The liver cold-preservation solution was taken for measurement of AST and ALT levels. The correlation among ATPase activity and lactic acid level was analyzed by SPSS 13.0 for windows.Results:The morphology in T group was improved in cell boundary versus C group on each time point. Dry weight to wet weight in T group was lower than in C group on 6h (P<0.05), but the difference was not significant on 24h (P>0.05). ALT levels in T group were lower than that in C group on 6h (P<0.05) and 24h (P<0.01). AST levels in T group was lower than that in C group on 2h (P<0.05),6h (P<0.01) and 24h (P<0.01). Na+-K+-ATPase activity in T group was higher than that in C group, the mean difference between two groups was significant on Oh (P<0.05) and 2h (P<0.05). Ca2+-ATPase activity in T group was higher than that in C group, the mean difference between two groups was significant on 2h (P<0.05). T groups increased lactic acid levels on Oh (P<0.01) and 2h (P<0.05) compared with C group, but there was no influence on LDH activity at the same time (P>0.05). There were no obvious differences on the levels of malondialdehyde between the two groups at each time point. A linear correlation between Na+-K+-ATPase activity and lactic acid levels(r=0.295, P<0.05) was found.Conclusions:Donor-pretreatment with Ulinastatin may protect the graft liver cell from ischemia injury in rats during cold preservation; the mechanism may be due to its promotion for cell glycolysis progress and its improvement in ATPase activity.