The Protein Levels And Its Clinical Significance Of Septin-9 And Clusterin In Peripheral Blood And Tumor Tissue Of Epithelial Ovarian Cancer Patients

OBJECTIVE:To evaluate septin-9 and clusterin protein levels in the peripheral blood samples and tumor tissues from epithelial ovarian cancer patients, and to explore its clinical significance.METHODS:The peripheral blood samples were obtained from 137 epithelial ovarian cancer patients,12 borderline ovarian tumor patients,10 benign ovarian tumor patients,41 benign pelvic lesion patients and 58 healthy women. The septin-9 and clusterin protein levels in the plasma were measured by enzyme-linked immunosorbent assay (ELISA). The tissue samples were obtained from 54 epithelial ovarian cancer patients,8 borderline ovarian tumor patients,8 benign ovarian tumor patients. The septin-9 and clusterin protein levels in the tissues were measured by immunohistochemistry (IHC).RESULTS:The mean levels of plasma septin-9 in epithelial ovarian cancer patients or benign pelvic lesion patients were significantly higher than that in healthy women (P=0.002, P=0.007). The mean levels of plasma septin-9 in epithelial ovarian carcinoma patients with tumor family history or distance metastasis ware significantly higher than of patients without (P=0.040, P=0.025). The mean level of plasma clusterin in epithelial ovarian cancer patients was significantly higher than that in healthy women (P=0.021). The septin-9 positive ratio of tissues in epithelial ovarian cancer patients or borderline ovarian tumor patients were significantly higher than that in benign ovarian tumor (p=0.007).CONCLUSION:The plasma protein levels of septin-9 and clusterin increase in peripheral blood of ovarian cancer patients. In epithelial ovarian carcinoma, the plasma level of septin-9 is correlated with distance metastasis of the tumor. The septin-9 protein level increases in tumor tissues of ovarian cancer patients.