The Systematic Study Of Compound Tablets Of Gou-teng Jiang-ya-ning About Two-kidney, One Clip Renal Hypertensive Rats Through The Effect Experiment And Clinical Research Based On Renin-angiotensin Aldosterone System

Objective:This research through the observation of compound tablets of Gou-teng Jiang-ya-ning in two-kidney,one clip renal hypertensive rats experimental study of the effect of primary hypertension Yin deficiency and yang excess syndrome, blood stasis patients for clinical research block,discusses the possible mechanism of compound tablets of Gou-teng Jiang-ya-ning.Methods:Clinical studies:will diagnosis of primary hypertension,levelland 2,the risk stratification of low, medium and high risk,traditional Chinese medical. primary hypertension patients with Yin deficiency and yang excess syndrome, blood stasis 122 randomly points into the treatment group and control group, the treatment group give compound tablets of Gou-teng Jiang-ya-ning,the control group piece, give medicine to enalaprilat.4 weeks after two groups of patients observe the change of signs and integral, blood pressure, blood pressure success rate, ejection fraction, left room, left ventricular Angâ… , Angâ…¡.Experimental study:(1)grouping:adopting healthy wister rats,randomly divided into two groups,the blank group with only 12,the experimental group with only 60,and the experimental group is divided into 12only with fake operated and 48only with operated.Will Wister rats surgical clipping left renal artery,set up two renal a clip renal vascular hypertension model of rats,the fake operated groups with the same procedure,but not placed silver clip cause renal artery stenosis.The operared rats screening 2weeks after,4weeks later tail arterial pressure to 150mmHg above 40mouse selected,according to the blood pressure level randomly divided into group A (model group),group B (compound tablets of Gou-teng Jiang-ya-ning),group C (telmisartan group),group D (enalaprilat group),Group E (the fake operated group),the group F for the blank group.(2)Each group measure blood pressure three times a week, take average;Observing four weeks later, using Enzyme-linked immunosorbent assay spectrometry Angâ… and Angâ…¡in plasma,serum ALD, ACE, ET-1, NO content; Observe heart, left kidney, thoracic aortic morphological and ultrastructural changes; Adopt enzyme-linked immunosorbent spectrometry in AT1R-AT2R, myocardial ATIR-Ab, by PCR detection content with thoracic aortic of myocardial AT1R mRNA, PMCA1 mRNA expression; Adopt Western blot test AT2R protein expression of myocardial.Statistics processing adopts 17.0 software SPSS analysis.Results:The results of clinical trials:compound tablets of Gou-teng Jiang-ya-ning can reduce hypertension Yin deficiency and yang excess syndrome, blood stasis block level, and reduce the patient's blood pressure,improve patients'blood pressure success rate,reduce the symptoms and signs integral Angâ… ,Angâ…¡level.Experiment results:(1)The two renal a clip made after surgical treatment of mould rat systolic blood pressure (SBP) blank group,significantly higher than the fake operated group(P<0.05).(2)After four weeks compared to medicine,compound tablets of Gou-teng Jiang-ya-ning can reduce renal vascular two renal a clip of hypertensive rats blood pressure (P<0.05). After treatment the compound tablets of Gou-teng Jiang-ya-ning group compared with model between in reducing Ang I,AngH,ALD,ACE,ET-l,rise NO are statistically significant differences (P<0.05);Can reduce AT1R,AT1R-Ab,AT2R in myocardial content;adjust PMCAlmRNA,AT1RmRNA in the myocardial and the thoracic aorta in expression;Adjust the AT2R proteins in myocardial expression.Conclusion:compound tablets of Gou-teng Jiang-ya-ning can reduce hypertension Yin deficiency and yang excess syndrome,blood stasis block the patient's blood pressure level;Raise blood pressure,improve the patients increase signs and integral;Ang I,Ang II lower levels.compound tablets of Gou-teng Jiang-ya-ning in 2K1C-RHR has significant antihypertensive effects,the function stability,lasting.compound tablets of Gou-teng Jiang-ya-ning can reduce Ang I,Ang II plasma,and serum ALD,ACE content,the role of RAAS has blocked and similar ACEI kind of antihypertensive effect.compound tablets of Gou-teng Jiang-ya-ning can make 2K1C-RHR serum NO content increased,ET level reduced,make both the proportion between equilibrium.compound tablets of Gou-teng Jiang-ya-ning could obviously decrease in the thoracic aorta 2K1C-RHR the film thickness of 2K1C-RHR chest,aortic vascular remodeling processes have certain inhibition.compound tablets of Gou-teng Jiang-ya-ning can significantly improve the 2K1C-RHR vascular endothelial dependence,restrain the vascular remodeling diastolic function;Have certain inhibition myocardial hypertrophy and LVH role.compound tablets of Gou-teng Jiang-ya-ning can reduce AT1R,AT2R in the myocardium,turn AT1R-Ab,AT1R mRNA,content of PMCA1 mRNA with thoracic aorta in myocardial AT2R protein expression,reduce the myocardial expression,with similar ARB kind of antihypertensive effect.