The Comparative Research On Different Mechanisms Of Tonifying Kidney Between Kidney-qi Bolus And Yougui Bolus Targeting At Function Of Reproduction And Renin-angiotensin-aldosterone System

This paper first systematically explains the two physiological functions of "the kidney controlling reproduction" and "the kidney controlling water metabolism" etc on the angle from literature. We discuss the fundamental conceptions of "kidney-JING" "kidney-QI" "kidney-YIN""kidney-YANG" which exist lots of controversy on the angle from historical origin.We also take it as emphasis that compare Kidney-qi Bolus with Yougui Bolus which both of are famous formula of tonifying kidney about effects and formulation,and contrast them on effects,indications,historic and contemporary herbal use.According to the analysis as above,we propose a hypothesis:Kidney-qi Bolus, whose target axis is chiefly located in hypothalamus-pituitary-kidney (HPK), is mainly used to recover the kidney controlling water metabolism. In contrast, Yougui Bolus, whose target axis is chiefly located in hypothalamus-pituitary-didymus(HPD), is mainly used to recover the function of the kidney storing the essence and controlling reproduction.We carried out animal experiments research,in order to obtain the further acknowledge of "the kidney controlling reproduction" and "the kidney controlling water metabolism"of traditional Chinese medicine,at the same time we tried to reveal preliminary mechanisms of tonifying kidney.Objective:Referring to available models of kidney-Yang deficiency syndrome, induced by " fatigue excess, intemperate sexual intercourse " at the point of view of etiology and "Cidomycin" at the point of view of pathology,we we will make a model of rats of renal deficiency.This paper aims to reveal different mechanisms of tonifying kidney between Kidney-qi Bolus and Yougui Bolus targeting at function of reproduction and renin-angiotensin-aldosterone system. Methods:It is induced by forced to swim everyday, College effects and Cidomycin to establish the rat model with "fatigue excess, intemperate sexual intercourse" of kidney-Yang deficiency syndrome. The suspension of Kidney-qi Bolus and Yougui Bolus by gavage is used to treat it.Adoptting technology of enzyme immunoassay and cellular tissue chemistry, we determine indicators in each group mouse,including sperm quality,testosterone, follicle-stimulating hormone,lymphatic system,renin,angiotensin II and aldosterone.etc.Results:1.The observation of change of the general condition showed that the mouse model of kidney-Yang deficiency syndrome caused by overfatigue and sexual overstrain was established.In the model group with kidney-Yang deficiency syndrome,the weight of testis,the amount and motility of sperm were lower than those in the control group,which reduced survival rate and increased deformity rate. Both Kidney-qi Bolus and Yougui Bolus resulted in increasing weight of testis,the amount and motility of sperm,so as to the higher survival rate and lower deformity rate.2.The process of establishing model had no significant effect on renin,but raised the level of Ang II and ALD.After the treat of Kidney-qi Bolus and Yougui Bolus,the higher level of Ang II didn't change.It suggested that Kidney-qi Bolus may decrease the level of ALD.Although the ALD of the test group with Yougui Bolus indicated declining tendency,it made no sense in Statistics.3.During the process of establishing model,the level of T,FSH and LH had declined.Both the treat of Kidney-qi Bolus and Yougui Bolus raised the three indicators at different degrees,but the effect of the latter was better.Conclusion:The mouse model of kidney-Yang deficiency syndrome is successfully established by "overfatigue, sexual overstrain and Gentamycin",which manifests spermatogenesis failure and part of RAAS hormone disorder. With Kidney-nourishing therapy by Kidney-qi Bolus and Yougui Bolus, above-mentioned function has been improved,and the latter's effect is better.This paper reveals that the recovery to the function of "the kidney controlling reproduction" of Yougui Bolus is slightly better than that of Kidney-qi Bolus.Meanwhile,that Kidney-qi Bolus makes significant influence on the level of ALD in RAAS suggests its effect on "the kidney controlling water metabolism" is better to a certain extent.