Research On The Transcriptome Features And Symptom Pattern Of Kidney-Yang Deficiency Syndrome Sufferring From Erectile Dysfunction

Objective:(1) With the technology of modern data mining, To analyze symptom characteristics of Kidney-Yang Deficiency syndrome Patients suffering from erectile dysfunction. And the symptom material are all from literature search and clinical records. (2) To explore the molecular biology of ED Kidney Yang Deficiency syndrome by microarray approach.Methods:(1) Literature and clinical research:study include:ED Kidney-Yang Deficiency syndrome of all relevant literature data of the "Chinese Medical Classics ",medical cases Clearly describe the ED Kidney-Yang Deficiency syndrome in Full-text Database of Chinese Academic Journals, investigation and collection of clinical cases of ED Kidney-Yang Deficiency syndrome in Affiliated Hospital of Chengdu University of Traditional Chinese Medicine and Sichuan Reproductive Hospital. Search Filter out the above symptoms data to take a normalization, and characteristics of the data requirements and data mining techniques to establish the appropriate rules, and build a database for ED Kidney-Yang Deficiency syndrome symptoms, (with a typical non-kidney yang deficiency symptoms to establish a database, statistical analysis for comparison purposes.) The use of statistical methods, including multiple regression, frequency analysis, cluster analysis, association rules, combined with Chinese medicine theory, the symptoms of ED Kidney-Yang Deficiency syndrome characteristics and composition of the regularity layers in depth analysis.(2)Transcriptome research:Select 3 cases of typical ED Kidney-Yang Deficiency syndrome patients from clinical collected cases and 3 healthy volunteers were carried out explore the transcriptome features of ED Kidney-Yang syndrome by microarray approach. Fasting venous blood drawn experimenter 10ml, cracking red blood cells, white blood cells were isolated, Trizol extraction of total RNA, total RNA and quantitative, quality inspection. IVT will be carried out in vitro expansion of total RNA, using cy-3 labeled amplified CDNA, a single standard method and agilent 4* 44k for the entire human cDNA microarray hybridization, washed, dried, data extraction, normalization processing. SAM software selection of differentially expressed genes, cutoff range:P< 0.01, FC> 2.Results:(1) Rely on data mining and statistical analysis combination with other methods to extract the ED Kidney-Yang Deficiency syndrome 22 most common symptoms, and based on statistical data to sort. Among them,4 main symptoms:ED, lumbar genu ache, Chills limb cold, tongue and pulse(pale and fat tongue, thin and weak pulse); 7 secondary symptoms:body tired fatigue, listlessness, waist and back chills, nocturia, dizziness, loss of libido, pale complexion; 12 general symptoms:drop after the urine, spermatorrhoea, infertility, anorexia eat less, nocturnal emission, coldness of genitals, abdominal pain, diarrhea, abdominal distention, semen cold, less semen, thin.(2)Selected 1396 differentially expressed genes by transcriptome research on ED Kidney-Yang Deficiency syndrome. The abnormal expression of these genes is closely related to the main symptoms and signs of ED Kidney-Yang Deficiency syndrome.These genes mainly related to:Protein serine/threonine phosphatase complex, regulation of metabolic process, intracellular organelles, macromolecular metabolic process, transcription regulator activity, ribonucleoprotein complex, regulation of molecular function, T cell selection, transcription cofactor activity, positive and negative regulation of metabolic process, biosynthetic process, multicellular organismal homeostasis, regulation of growth, cellular metabolic process, viral reproductive process, leukocyte activation, and so on.In the differentially expressed genes,10 protein serine/threonine phosphatase complex gene expression is most prominent, of which five genes were upregulated and 5 genes were downregulated. Differences in genes involved in a variety of functional proteins and metabolic pathways. The major signaling pathway involved in are:Alzheimer's disease, metabolic pathways, N-Glycan biosynthesis, Ubiquitin mediated proteolysis, Wnt signaling pathway, Insulin signaling pathway, RIG-I-like receptor signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Calcium signaling pathway.The abnormal expression of genes and signaling pathways are closely related to main symptoms and signs of ED Kidney-Yang Deficiency syndrome. And it suggests that sex hormone metabolism is low, lack of energy synthesis, metabolism and immune dysfunction in those patients.Conclusion:(1) Through the literature, the author initially reveals the symptom characteristic, the rule of combination and the reference diagnostic method about ED Kidney-Yang Deficiency syndrome, perfects for ED Kidney-Yang Deficiency syndrome's understanding.(2) Through the transcriptome experimental study, we found that the 10 protein serine/threonine phosphatase complex genes expression is most prominent, of which five genes were upregulated and 5 genes were downregulated, in the differentially expressed genes. This is some difference from former related research, maybe suggest ED Kidney-Yang Deficiency syndrome may occur closely related with the protein serine/threonine phosphatase complexe.(3) ED Kidney-Yang Deficiency syndrome is a complicated pathophysiologic process involving multiple proteins and metabolic pathways. In the relevant signaling pathway, involving multiple signaling pathways, suggesting that the incidence of ED Kidney-Yang Deficiency syndrome are the results of many signal pathways cooperated. Which, Wnt signaling pathway, MAPK signaling pathway, Ubiquitin mediated proteolysis and Alzheimer's related neurodegeneration may play an important role in the foundation.