Effects Curcumin Having On TGF-β/Smads Pathway Of Human DU-145 Prostatic Cancer Cells

Objectives:To observe the efficacy Curcumine having on TGF-(3/Smads pathway of human DU-145 prostatic cancer cells in hormone-independent prostatic cancer, and to accordingly observe the principle of it restraining DU-145 cells.Methods:Cell cultivation was adopted with different groups using 12.5μmol/l,6.25μmol/l and 3.125μmol/l Curcumine concentrations on DU-145 cells for 48 hours each. The DU-145 cells'morphology was observed under optic microscope. The multiplication of DU-145 cells was measured by MTT method. Both ELLISA and RT-PCR methods was used to measure the concentrations and expressions of TGF-β,SMAD2, SMAD4 and SMAD7.Results:DU-145 cells'morphologic changes were shown with number decreasing, volume reduction, chromatin coagulation and nucleus contraction after 48 hours'influence Curcumine concentrations had on them. A certain quantity-effect relation was shown in each concentration group. The values of OD of TGF-β,SMAD2 and SMAD4 were increased by the concentration groups measured with ELLISA but SMAD7 were lowed. The expressions of TGF-β,SMAD2 and SMAD4 were increased by the concentration groups measured with RT-PCR but SMAD7 were lowed. The two methods of measurement showed the same results.Conclusions:To intervention TGF-β, SMAD2, SMAD4 and SMAD7 of human DU-145 prostatic cancer cells,curcumine can activation or regulate TGF-β/Smads pathway of them,what could be the important mechanism of curcumine restraining DU-145 cells...