| Objective:Clinical study by Yang Xin Tang on the "little blood deficiency, blood running weakness, venae stasis, mind dystrophy" as the pathogenesis of unstable angina (unstable angina pectoris, UA) patients the efficacy and mechanism For Integrative Medicine to develop a comprehensive treatment program UA provide an objective basis. Yang Xin Tang observed UA patients on the impact of metabolomics to explore its possible mechanism of action, the objective for the UA desertification syndromes explored.Methods:A randomized, controlled method 103 patients were divided into experimental group (Yang Xin Tang plus conventional therapy), the control group (conventional therapy), continuous observation for 28 days, on TCM syndrome, angina symptoms, electrocardiogram, Reduced rate of nitroglycerin stop, tongue, quality of life, and follow-up statistics on the changes.The use of liquid chromatography-mass spectrometry technique in 10 healthy volunteers,10 patients with UA were detected in plasma before and after medication, the use of partial least squares discriminant analysis method to observe the UA patients before and after medication changes in composition of plasma metabolites, Metabolomics perspective from Yang Xin Tang on the impact of UA patients, and in the healthy group and disease groups looking for metabolomics markers.Results:1 total effective rate of angina pectoris study group 94.33%,92% of the control group no significant difference between the two groups (P> 0.05).2 TCM symptoms test group the total effective 98.11%,94% of the control group no significant difference between the two groups (P> 0.05).3 ECG test group the total effective rate 69.81%,64% of the control group the difference was statistically significant (P<0.05).4 nitroglycerin stopped by 62.26% rate of the experimental group and control group 50% of the difference was statistically significant (P<0.01).5 Seattle Angina Questionnaire integral dimension in AS the difference between two groups was statistically significant (P<0.05).6, tongue, tongue coating properties, the tongue (tongue questionnaire) statistical difference was statistically significant (P<0.05).7 tongue color (R/B/G color flux) Statistical difference was statistically significant (P<0.05).8D, M, G among the three groups of PCA analysis showed that all three groups of samples concentrated on the score plot of the oval (95% confidence region) of the four regions, M samples of the group There are some differences, showing the relative dispersion of sample bias 8,9,10D, three basic groups can be separated; M (before treatment) group and G (after treatment) can be separated between the two groups basic; 9 D group M group compared metabolic end products found:disease group than in the healthy group elevated metabolites ceramide, Glycocholic acid, not acid, lithocholic acid, leukotriene B4 and so on.Conclusion:1 test group and control group UA group could significantly improve symptoms in patients with Chinese medicine, symptoms of angina, ECG, nitroglycerin stopped by rate. In ECG, the rate of nitroglycerin test group stopped by the degree of improvement than the control group.2 experimental groups and control group could significantly improve the quality of life of patients UA, in Seattle Angina Questionnaire angina stability (AS) the dimension of the experimental group than the control group improvement.3 This study Tongue scale by the experimental group and control group that could significantly improve the tongue in patients with UA, in the tongue, tongue, tongue three degree of improvement in the test group than the control group, The study by digital photography analysis of the tongue did not find experimental group and control group of red /green/blue three color components have changed before and after treatment.4 metabonomics:A study by high performance liquid chromatography mass spectrometry observed in healthy controls and disease groups, Yang Xin Tang UA before and after treatment of the disease in patients with different metabolomics profiles,differences in metabolism between the two groups, there are different pathways that can interfere with UA Yang Xin Tang plasma metabolites or metabolic pathways Erzhi metabolic end Product changes.5This study found that patients with UA may exist: typical signs of metabolites of leukotriene B4, Glycocholic acid, do not lithocholic acid, ceramides.
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