| Objective:To observe the effective regulation of acupuncture-moxibustion on ST36 for post infectious irritable bowel syndrome in rats, and investigate the method and working mechanism of different organizational structure for colonic visceral hypersensitivity and gut disfunction. To search the scar-producing moxibustion in classical documents, and draw the technique of radio frequency into acupuncture.Methods:Wistar famale rats have been used in this study, and then the animals had been randomly divided into 5 groups:control, post infectious irritable bowel syndrome model, electro-acupuncture, scar-producing moxibustion and radio frequency group. The needlepoint of electroacupuncture and radio frequency was penetrated in tibialis anterior, and the moxa cone was puted on skin. Post infectious irritable bowel syndrome was induced by intracolonic instillation of trinitro-benzene-sulfonic acid in diarrhea model group and the control group was using same method by intracolonic instillation of normal salt solution. All experiments were conducted at the 28th day of intracolonic instillation. The point ST36 was used in this study for 15min in electro-acupuncture or 3 moxa cone with 40mg in scar-producing moxibustion or 75℃and 200sec in radio frequency. The scores of abdorminal withdrawal reflex induced by colorectal distention for visceral hypersensitivity was used in this study at the 33th day of intracolonic instillation, and total gastrointestinal transit times was used for gut disfunction at same day, also mean tone and frequency or maximum amplitude of longitudinal colonic smooth muscle strips was used like just before to observe the effective regulation of acupuncture-moxibustion on ST36. The rats concentrations of substance P and 5-HT and calcitonin gene-related peptide in plasma were detected by a double-antibody sandwich enzyme-linked immunosorbent assay to investigate working mechanism of acupunture-moxibustion.Result:1. The scores of abdorminal withdrawal reflex (AWR). The scores of model group was more than control group signif icantly(P<0.005), scores of EA or SP-Moxi or RF group have decerased significantly (P<0.05-0.005), and then did not change significantly among last 3 groups (P>0.05), but it was on significant change between the group RF and cotrol in 20mmHg by colorectal distention (P>0.05)2. The histopathology of acupoint ST36 after acupuncture and moxibustion. There are some inflammatory cells and a few mesothelium hyperplasia after eletroacupuncture, tissue necrosis of epithelium and chronic inflammation after scar-producing moxibustion, and tissue necrosis of tibialis anterior after radio frequency. 3. Total gastrointestinal transit function. The times of activated carbon discharged of model group was less than control group significantly (P<0.05), times of EA or SP-moxi or RF group were longer significantly (P<0.05-0.01) than model group, and then it was better significantly between RF and the other two group (P<0.05)4. Mean tone of longitudinal colonic smooth muscle strips. The tone of model increased significantly than control (P<0.05), by means of acupuncture-moxi bust ion therapy on ST36 acupoint, The tone decreased significantly (P<0.05-0.01),and then did not change significantly among last 3 groups (P>0.05). In Acetylcholine solution, the mean tone increased gradually due to more and more concentration, after 10-6, it increased significantly (P<0.005) than basic in group of control and model, but after 10-5, it began to increase significantly(P<0.05-0.005) than basic in group of EA and SP-Moxi and RF, and then did not change significantly among last 3 groups (P>0.05)5. Maximum amplitude of longitudinal colonic smooth muscle strips. Group of model increased significantly than control (P<0.005),the maximum amplitude in group of EA and SP-Moxi and RF decreased significantly (P<0.05-0.01), and then it decreased significantly between RF and the other two group (P<0.05)In Acetylcholine solution, the maximum amplitude increased gradually due to more and more concentration until 10-8, its maximum was in 10-8 Acetylcholine solution. After 10-7, the maximum amplitude decreased gradually due to tetanic contraction of strips. In 10-8, it increased significantly (P<0.05-0.01) in group of control and model, After 10-6, it decreased significantly than basic (P<0.025-0.005).But After 10-5, it decreased significantly than its basic in group of EA and SP-moxi (P<0.05-0.005), After 10-6, it decreased significantly than its basic in group of RF (P<0.05-0.005).Compared among 3 groups, the group of RF decreased significantly than the group of EA and SP-moxi in 10-9 and 10-8 (P<0.025-0.01), the group of RF decreased significantly than the group of EA in 10-7 (P<0.01), no change significantly among 3 groups in 10-6 and 10-5 (P>0.05), the group of SP-moxi and RF decreased significantly than the group of EA in 10-4 (P<0.01)6. Mean frequency of longitudinal colonic smooth muscle strips. It did not effect the mean frequency among 5 groups (P>0.05). In Acetylcholine solution, the mean frequency increased gradually due to more and more concentration until 10-6. After 10-5, the mean frequency decreased gradually due to tetanic contraction of strips. After 10-5, it changed significantly (P<0.005) in group of control, but in 10-6, it changed significantly than basic (P<0.005), the group of RF did not effect. Contrasted among 3 groups, there were not much difference in Acetylcholine solution concentration from 10-9 to 10-5 (P>0.05), but in 10-4, the group of SP-moxi and RF decreased significantly than the group of EA (P<0.025-0.005), the group of SP-moxi changed significantly than the group of EA and RF in 10-4 (P<0.025).7. The plasma 5-hydroxytryptamine and substance P increased significantly than befor in PI-IBS model rats (P<0.01-0.005), calcitonin gene-related peptide decreased significantly than beforc. The plasma 5-HT and SP decreased significantly than before in the group of EA and SP-moxi and RF rats, CGRP increased significantly than before (P<0.05-0.005). there were not much difference contrasted among last 3 groups, the RF improved than control group in SP and CGRP,but did not still recover (P<0.05-0.025)8. The rule of variation tendency of mass-temperature-time in moxa cone. The maximun temperature or moment or duration of moxa-cone were positive in pertinency beween mass and others.9. The rule of variation tendency of temperature-time. The curve of temperature-time of one moxa cone was a single-crest wave, it consisted of slowly rising stage and quickly rising stage and slowly descent stage and quickly descent stage.Conclusion:1. It can treat colonic visceral hypersensitivity and gut disfunction of IBS with acupuncture and moxibustion.2. By means of regulation of tone and amplitude of longitudinal colonic smooth muscle strips, it can treat gut disfunction of IBS with acupuncture and moxibustion.3. The skin of ST36 with scar-producing moxibustion can treat colonic visceral hypersensitivity and gut disfunction of IBS, but anterior one is better than post.4. The tibialis anterior of ST36 with radio frequency can treat gut disfunction and colonic visceral hypersensitivity of IBS, but anterior one is better than post.5. By means of ST36 of IBS with acupuncture and moxibuston,5-HT, SP and CGRP were intermedia of disturbed nerve-internalsecretion-immunity network.6. The maximun temperature or moment or duration of moxa-cone were positive in pertinency beween mass and others. Curve of variation tendency of temperature-time was a single-crest wave, it consisted of slowly rising stage and quickly rising stage and slowly descent stage and quickly descent stage.
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