Clinical Significance Of Genetic Abnormalities Detected By Interphase Fluorescence In Situ Hybridization In Multiple Myeloma And Chronic Lymphocyte Leukemia

Object:To investigate the clinical significance of the chromosomal changes in Chinese MM patients.Method:Interphase fluorescence in situ hybridization (FISH) on bone marrow (BM) cells was performed in 166 enrolled MM patients. Relationships between chromosomal abnormalities and clinical features, response to therapies and prognosis were analyzed.Results:As a result of FISH,88.0%(146/166) patients had chromosome changes. The incidences of each probe were IGH 58.4%, t(11;14) 25.9%, t(4;14) 17.5%, t(14;16) 7.2%, undetermined t(14q32) 7.8%. RBI 55.4%, D13S319 53.6%, amplq21 49.4%, and del17p13 25.3%. Each cytogenetic abnormality was significantly correlated to clinical features. Treatments with bortezomib gave patients a notably higher CR+VGPR rates, especially for patients with del13q14 (p=0.004). The media PFS of patients with dell7pl3 was 4.9 months (vs 8.9 m,p<0.001). The media PFS of patients with amp1q21 was 5.9 m (vs 8.8m, p=0.002). And t(14;16) was 3.0 m (vs 7.1 m, p=0.006), del13q14 was 6.7 m (vs 8.6 m, p=0.043). Among patients with del13q14, the ones with dell7pl3 had a shorter median PFS(4.6m vs 8.0m,p=0.002). And among patients without del17p13, there's no significant difference of PFS between patients with and without del13q14.Conclusion:Chinese MM patients had a higher dell7p13 rate compared with people in western countries. The other prevalence of chromosomal abnormalities of MM patients was similar in Chinese and other people. Cytogenetic changes were associated with patients' responses to therapies and prognosis. Partâ…¡Comparison of cytoplasmic immunoglobulin light chain FISH and normal FISH on detecting cytogenetic abnormalities in multiple myeloma patientsObject:To compare the positive rates of cytogenetic changes detected by cIg FISH and normal FISH in myeloma patients.Method:To perform cIg FISH and FISH on same patient's sample and compare the positive rates.55 myeloma patients were analyzed in all.Results:As a result of cIg FISH,58.2%(32/55) patients had del13q14 compared with 41.8%(23/55) by normal FISH. And 52.7%(29/55) patients had amplq21 by clg FISH compared with 41.8%(23/55). Among the patients with plasma cells<30%, clg FISH found 51.9%(14/27) del13q14 patients compared with 22.2%(6/27) by normal FISH (p=0.024). And 55.6%(15/27) amplq21 patients compared with 29.6%(8/27) (p=0.054). But in patients with plasma cells>30%, the positive rates showed no significant difference between clg FISH and normal FISH.Conclusion:Among the patients with plasma cells<30%, cIg FISH improved the detection rate of cytogenetic abnormalities significantly. Partâ…¢Clinical significance of cytogenetic abnormalities detected by interphase fluorescence in situ hybridization in chronic lymphocyte leukemia patientsObject:To investigate the clinical significance of the chromosomal changes in Chinese CLL patients.Method:Interphase fluorescence in situ hybridization (FISH) on peripheral blood cells was performed in 94 CLL patients. Relationships between chromosomal abnormalities and clinical features was analyzed.Results:As a result of interphase FISH,81.9%(77/94) patients had chromosome changes. 35 patients had more than one abnormalities. The most frequent change was del13q 14, the incidence of that was 55.3%(52/94). The next was trisomy 12, which was observed in 25.5%(24/94) patients. The incidence of del11q22 was 19.1%(18/94), del17p13 was 17.0%(16/94). Most del11q22 or/and del17p13 patients were in Rai stage 3 and 4, and in Binet stage C. Del17p13 had a significant relationship with Rai stage(r=0.310,p=0.003) and Binet stage(r=0.229, p=0.032). The percentage of delllq22 cells was significantly correlated to Binet (p=0.001) stage systems.Conclusion:The prevalence of chromosomal abnormalities of CLL patients was similar compared to literatures. Cytogenetic changes were associated with patients' clinic stages.