The Experimental Study On Sps To Tumor Metastasis-related Genes Expression

Objective:To study the SPS which has an effect on BALB/c mice bearing S180tumor metastasis related genes CD44,MMP-9, TIMP-1,AMF mRNA and nm23-H1protein, to observe the SPS on angiogenesis and anti-tumor effect, to explore the SPS refrain tumor metastasis and its mechanism furthermore.Methods:1. Detect the CD44and MMP-9, TIMP-1, AMF mRNA expression of BALB/c mice tumor tissue by Real Time PCR assay2. Detect the expression of nm23-H1protein of tumor tissue by Western blot assay.3. Chicken embryos chorioallantoic membrane assay to observe angiogenesis number and size of the change.4. Immunohistochemical assay to detect the mice tumor tissue VEGF protein and MVD changes.5. ELISA assay to detect serum IL-12, IL-10and TNF-α content.Results:1. Real Time PCR detection,the SPS role in tumor-bearing mice, tumor tissue of CD44,MMP-9,AMF mRNA expression was decreased and TIMP-1mRNA expression was increased.2. Western blot analysis found that the nm23-H1protein content increased in the tumor tissue of mice with the reaction of the SPS.3. SPS mid-dose group after the intervention of the SPS in the chicken embryos chorioallantoic membrane angiogenesis, the number and size compared with the model control group decreased (P<0.05),SPS high-dose group compared with the model control group was significantly reduced (P<0.01)4. Immunohistochemical methods detected the SPS mid-dose groups VEGF expression in tumor tissue was significantly decreased (compared with model group P<0.01), MVD reduction is extremely obvious (P<0.001); VEGF positive expression rates and MVD reduction in the SPS high-dose group of mice tumor tissue are extremely clear (P<0.001)5. ELISA examination found that compared with the model group, the mid-dose, high dose of the serum IL-12and TNF-α levels increased very significantly (P<0.001), IL-10decreased very significantly (P<0.001)Conclusion:1. SPS can inhibit tumor tissue of mice CD44, MMP-9, the AMF mRNA expression, promote TIMP-1mRNA expression. SPS can inhibit the adhesion, degradation, and motion of tumor metastasis.2. SPS can restrain tumor metastasis by promoting the expression of nm23-H1in tumor tissue of mice3. SPS can inhibit the generation of the chicken embryos chorioallantoic membrane vascular.4. By inhibiting VEGF expression and reducing MVD, SPS can restrain the growth of blood vessels in the tumor tissue, make tumor growth and transfer under control.5. SPS can increase the expression of IL-12, reducing the expression of IL-10to adjust the Th1/Th2shift, and to promote TNF-α expression.