Experimental Study Of Adipose-derived Stem Cells Transplantation For Myocardial Infraction In Sprague Dawley Rat

[Objective] To investigate the feasibility inducing adipose-derived stem cells into myogenic cells in vitro and a potential treatment of ischemic heart disease with transplantation of adipose-derived stem cells.[Methods]1Adipose-derived stem cells are taken from Sprague Dawley (SD)rat's mesenteric adipose tissue and cultured, third-passaged cells were exposed to10μmol·1-1dilutions of5-azacytidine for24hours. Transmission Electron microscopy, Scanning Electron microscopy, immunohistochemistry were used to demonstrate the characteristics of the cells.2ADSCs were incubated with culture medium containing40μg/ml USPIO and1.5μg/ml poly-1-lysine (PLL) for24h. The distribution of iron particles in cells was determined by Prussian blue staining and transmission electron microscopy (TEM).3A model myocardial infarction was created by ligation of the distal left anterior descending artery in SD rat. The labeled ADSCs were injected transthoracic into the myocardium. In vivo.4The infraction area was assessed by Micro-PET and MR. MR imaging was performed with FIESTA Cine, FSPGR Cine and2D MDE sequences for all groups. The left-ventricular end-diastolic volume (LVEDV), left-ventricular end-systolic volume (LVESV) and left-ventricular ejection fraction (LVEF) were calculated on Report Card Workstastion to assess the cardiac function. Postmortal study was carried out to determine the distribution of USPIO particles in the myocadium with Prussian blue stain.[Results] The myofiber in adipose-derived stem cells was demonstrated by transmission electron microscopy after ADSCs exposed to5-aza, which was stained by immunohistochemistry of anti-myoglobin antibody. The cardiac function of the Sprague Dawley rat was improved and the infraction area was reduced after the transplantation of adipose-derived stem cells. [Conclusion] Adipose-derived stem cells can differentiate into myogenic cells in vitro after5-azacytidine exposure. Transplantation adipose-derived stem cells may improve cardiac function of ischemic heart disease and shrink the infraction area of the heart in Sprague Dawley rat.