Candida Albicans Sensitive Strains Produce Adaptive Mutation Resistant To Fluconazole

Candida albicans is the most frequent human opportunistic fungus. Theinfection caused by the pathogenic yeast is quickly increasing with the risingnumber of the immunodeficient population. Fluconazole, one of thetrianazole, came to use at the end of 90's. It is widely used to treat thefungus-infected, especially in the individuals whose systems are destroyed bypathogenic fungi. With the emerging and developing of the resistan strains,more doses have to be given to control the infection of Candida albicans.We choose the fluconazole-susceptible(MIC<0.5 μ g/ml) candidaalbicans clone-Y0l-09 clone(the standard wild clone) and culture it in theplates containing fluconazole. Finally, it may change into the strain whoseMiC is 32 μ g/ml. Having counted all the colonies growing in each plate, weimitate a curve according to the theory of caims. The curve shows anexpected distribution as the Poisson's fluctuation test. So according to thephenotype, we initially conclude that the main source of the fluconazole-susceptible candida albicans' adapive mutation to fluconazole.According to the figure of their knyotypes and the DNAfingerprnt(care-2) hybridizations we make a conclusion, that the Y0l-09strain(MIC<0.5 μ g/ml), the induced strain(MIC ≥ 32 μ g/ml), and thereverted strain(MIC also <0.5 μ g/ml) are the same strain. Anong the mainlyknown areidrug-resistance genes of the induced strains, only the CaMDRl-mRNA has increased, and its sequence has occurred severaI significant pointmutations. Overall, the candida albicans' susceptibilities to fluconazole candecrease with the fungus' adaptive mutation to the drug.