| The ecological distribution , composition and flora of the Fritillaria cirrhosa communities has been showed by the classification of cluster analysis and percentage of similarity. The communities of F.cirrhosa include Sibiraea angustata community ,Rhododendron litangense community , Salix sclerophylla community, Dasiphora fruticosa+Spiraea alpina eommunity+S.myrtilloides community, Polygonum viviparum+P. sphaerostachyum community. The research results will supply a base for the culture and protection of F. cirrhosa. The genomic DNA variations of 12 samples of Fritillaria were analyzed using random amplified polymorphism DNA (RAPD) in the present study. Twelve deeanuleotide arbitrary primers generated 104 RAPD bands with the size ranging from 100 bp to 1500 bp, 78 of these bands were polymorphic (75%). The similarities and genetic distances among them were analyzed with Jaccard's and Nei's methods respectively. The dendrogram based on the genetic distances revealed that the 12 samples could be classified into three groups ?a. Friti]laria unibracteata, P. cirrhosa, F. pz-zewa]skii b. F. taipaiensis, F. sp F. delavayi c. F. thunbergii var. chekiangensis, F. thunbergii , F. ussuriensis , F. pallidiflora, which agrees with the conventional taxonomic position. It showed that RAPD technique could be used for classifying and identifying at the level of species.
|
PDF Full Text Request