Study On The Phytochemistry Taxonomy Of Blumea DC

Blumea genus(Blumea DC.)is one of the most important of the Asteraceae(Asteracea)Inula Group(Inuleae)taxa,and also one of the most economically important plant groups Asteraceae,under the rich medicinal plant resources("modern Compendium of Materia Medica" and "China national Flora" collection contains 12 to15 species,accounting for 40% of the genus species ～ 50%),as well as a variety of chemical constituents remarkable and unique efficacy has been shown greater research and development potential,caused by many scholars of great interest.But its chemical constituents of the poor,the relationship between the two groups classified confusion,lack of effective means and methods of identifying,limiting its wide application to a great extent.Chemical plant taxonomy traditional chemical constituents of plants used in plant taxonomy,is of great value in elucidating the common components and characteristics of ingredients,relations between groups to clarify and expand related species,looking for the source and development of new drugs,rapid identification of medicinal herbs,etc.the present study intends to use chemical plant taxonomy TLC methods,GC method,LC method and other chemical constituents research methods,Blumea chemical constituents systematic study,to clarify terpenoids,flavonoids Ai distribution and evolution of the genus satisfied incense,explore the chemical composition of the differences between groups and between species,genus looking Blumea herbs rapid identification methods,expand Blumea medicinal plant resources and genetic resources,to lay the foundation.This article Blumea genus 10 species of medicinal plants were determined fingerprints,to further complement Blumea of medicinal plants of the genus.Includes the following three parts:1.TLC method Blumea medicinal plants for chemical taxonomic studyIn this study,the use of TLC,Blumea medicinal plants chemical taxonomic research.The Blumea dried medicinal plants,ultrasonic extraction methods,namely methanol,ethyl acetate as the extraction solvent,the experimental sample is processed,wherein the methanol extract eluent,methylene chloride: acetone(12: 1),the ethyl acetate extract eluent petroleum ether-ethyl acetate-chloroform(3: 1: 0.5),respectively,both record spots expanded Rf value,Rf value resulting spots were compared to discuss the results of the analysis.2.GC method Blumea medicinal plants for chemical taxonomic studyThe experiment Blumea medicinal plants in the fingerprint of established Blumea medicinal plants fingerprint.By GC method can detect a total of 42 peaks,and through fingerprint of camphor,isobornyl,L-borneol,xanthoxylin four chemical components were determined qualitative identification and content.Blumea established medicinal plant control fingerprint spectra showed that 10 kinds of herbs Blumea genus similarity there are some differences.3.HPLC method Blumea medicinal plants for chemical taxonomic studyThe experiment Blumea medicinal plants in the fingerprint of established Blumea medicinal plants fingerprints.Established by HPLC Blumea medicinal plant control fingerprints,and the fingerprints of the measured similarity evaluation for Blumea Taxonomy,medicinal plants and other genetic relationship to provide a reference.In this study,the use of TLC,GC,HPLC method Blumea medicinal plants were established fingerprint method is simple,accurate,reproducible,classification of medicinal plants of the genus is Blumea provided.