| In 1967, Christiaan Barnard led the team performed the world's first human-to-human heart transplantation, followed with America and Europe. Heart transplantation is currently the only clinical treatment for end stage heart disease patients. The number of the heart transplantation cases had decreased while 1994. One of the most important reasons is the lack of donor hearts because the preservation time of donor heart is too short. It is essential for successful heart transplantation to improve preservation donor heart safely and effectively. The prolonging of preservation time of donor heart could expand the donor hearts pool.A lot of researches confirmed that many effective extracts of Chinese herbs had effects of resisting ischemia-reperfusion(IR) injury and protecting myocardial. It is feasible that the effective Chinese herbs or extracts of Chinese herbs are added into conventional preserve solution to improve the effects of heart preservation, prolong the heart preserve time, and to expand Chinese herbs application.The objective of this research is: To select systematically Chinese herbs that have effects of resisting isochemia-reperfusion injury and protecting myocardial. To look for Chinese herbs that have the effects on heart preservation and prolong the preserve time of donor heart.Firstly, We established mice Langendorff heart perfusion method for preliminary selection of Chinese herbs. It is possible to measure left ventricular function including LVPSP, ?dp/dtmax, HR, RPP and CF of isolated heart. Stableness of the model had been confirmed by experiments. It is possible to study for heart preservation.Secondly, with mice Langendorff isolated heart perfusion model, we add the generally recognized intracellular cardial preservation solution-modified Euro-Col 1 ins solution (mECS) with: G. Lucidum Karst (Ji Sheng), Carthamas tinciorius L (Hong Hua), Acanthopanax Senticosus Harms (Ci Wu Jia), Saponins of panax notoginseng, PNS (Xue Sai Tong), Erigeron breviscapus Hand-Mazz, EbHM(Deng Zhan Hua), Huang Qi, radix angelicase sinensis(Dang Gui), Tetramethylpyrazine, TMP(Chang Xiong Qin), respectively. Mice hearts were preserved for 3hr, 7. 5hr, 9hr, respectively, under simple cold storage condition with mECS as control.After preserved for 3hr, Ji Sheng, Hong Hua, Ci Wu Jia, Xue Sai Tong, Deng Zhan hua all improved left ventricular function, including LVPSP,眃p/dtmax, HR, RPP, CF. Effectiveness of Ji Sheng, Hong Hua, Ci Wu Jia group was better than that of Xue Sai long and Deng Zhan Hua .After preserved 7. 5hr for mice hearts: Resurrect rate of Hong Hua group was significantly higher than that of control group (p<0. 01). Resurrect rate of Ci Wu Jia group was also significantly higher than that of group (p<0. 05). Resurrect rates of Ji Sheng and Deng Zhan Hua groups were higher than that of control group, but no statistics sense. Resurrect rate of Mice heats of Xue Sai long group was lower than that of control group.After preserved 9hr of mice hearts: Resurrect rates of Hong Hua and Ji Sheng groups were significantly higher than that of control group (p<0. 01, p<0. 05). Resurrect rates of Ci Wu Jia, Huang Qi and Chuan Xiong Qin groups were also higher than that of control group, but no statistics sense. As for resurrect rate of mice hearts, Hong Hua group was the highest, Ji Sheng group was the second highest, and Dang Gui group was lowest among the six groups. As for the heart beat regaining time, Hong Hua group was the lowest and Chuan Xiong Qin group was the second lowest among the six groups without statistical sense. Myocardial water contents of Ci Wu Jia and Hong Hua groups were significantly lower than that of mEC group(p<0. 01). CF of Dang Gui group was significantly higher than mECS group (p<0. 05).Secondly, The experiments were done with rat hearts used Euro-Collins solution adding Ji Sheng, Hong Hua, Ci Wu Jia, Huang Qi, Chuan Xiong Qin, and Dang Gui, respectively. Rat hearts were preserved for 20hr under simple cold storage condition with mECS compare with the study s...
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