Refractory Epilepsy Study On The Risk Factors And Pathogenesis

Objective:To explore the pathogenic mechanisms of intractable epilepsy by searching for the differential cell signal transduction-associated genes expression in intractable and non-intractable epilepsy rat brain using cDNA microarray.Methods: Intractable epilepsy and non-intractable epilepsy rat model were build. The total RNAs were isolated from the brain tissues, and were purified to mRNAs by Oligotex. Both mRNAs from the brains of the intractable and non-intractable epilepsy rats were reversely transcribed to the cDNA with the incorporation of fluorescent dUTP to prepare the hybridization probes. The PCR products of 4096 human genes were spotted on a chemical-material-coated-glass plates in array. DNAs were fixed onto the glass plate after series of treatments. The mixed probes were thenhybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues.Results: Among the 4096 target genes, 29 genes associated with cell signal transduction differentially expressed were identified,10 were up-regulated(34.48%)and 19 down-regulated(65.52%).Conclusion: cDNA microarray technology is an effective technique in screening the differentially expressed genes between intractable and non-intractable epilepsy rat brain. Disturbances of cell signal transduction play a role in the pathogenic mechanism of intractable epilepsy.