Correlative Study On TNFα Signal Transduction Apotosis And Human Temporal Lobe Epilepsy

[Objective]To study the relevance of TNFαtransduction cell apotosis and human temporal lobe epilepsy(TLE).[Methods] SP dyeing of Immunohistochemistry was used to detect the expression of TNFR-assosiated death domain protein(TRADD),apoptosis signal-regulating kinase 1(ASK1)and caspase 8 of human TLE group and contrast group,and their expression was analyzed. Neurons,gliocytes and microvasculars in the foci of TLE were observed by transmission electron microscope.[Results]The expression of caspase 8 in neurons of TLE group was increased than that in contrast group(P<0.05). The expression of caspase 8 in gliocytes of TLE group was obviously increased than that in contrast group(P<0.01). The expression of TRADD and ASK1 was not found in neurons and gliocytes of TLE group and contrast group.The expression of TRADD and ASK1 was obviously increased in microvascular endotheliocytes of TLE group than that in contrast group(P<0.01).The expression of caspase 8 was not found in microvascular endotheliocytes of TLE group and contrast group.Under transmission electron microscope,distinctive apotosis changes were found in neurons of TLE group,including the neurons decreasing in volume,nuclear pyknosis,increasing and marginal collection of heterochromatin,shrinkage of cytomembrane and nucleolemma,nuclear division,forming apoptotic bodies.Changes of microvasculars were also found.Those include microvasculars appear twisted and obstructed,or endotheliocytes swell,basement membrane increases in thickness or delamination.And astrocytes were found swollen.[Conclusions]In the temporal lobe tissue of patients with refractory TLE:1,TNFαtransduction cell apotosis may exist in neurons and gliocytes;2,TNFαtransduction cell apotosis exists in microvascular endotheliocytes;3,The molecular mechanism of TNFαtransduction cell apotosis between them may be different.