Chinese Medicine Intestinal Absorption Kinetics - Of Breviscapine Intestinal Absorption Kinetics

This reserch was studied on the Breviscapine intestinal absorption dynamics first-time with 3 methods : everted sac technique, body circulatory perfusion technique and Caco-2 cell method.The result of everted sac technique test showed the way of Breviscapine intestinal absorbtion is passive transport mechanism and maximum absorptivity varied from 13.1% to 16.9%.The result of body circulatory perfusion technique test showed the same absorption mechanism with Breviscapine .Maximum absorptivity varied from 15.41% to 16.38%, the absorption Ka was 0.0329~0.0358h-1,absorption-fraction per hour was0.0507~0.0546,Papp was 1.137x10-7~1.240x10-7, absorption t1/2 was 19.45h~21.70h and with no influence on intestinal bacterial flora.The result of Caco-2 cellular model manifested the transenterocyte movement of Breviscapine was passive diffusion,maximum diffusibility changed from 16.78% to 17.11%, diffusion -fraction per hour was 0.0833-0.0856, diffusion Ka was 23.59 x 10-7~24.76 x 10-7,diffusion t1/2 was 16.5h~17.33h.. Efflux test demonstrated no adverse efflux.Those results showed there were satisfied correlation among the three experiment tests. The absorption mechanism of Breviscapine was passive diffusion, characterized by long absorption t1/2 and poor absorption. Relative documents and reserches illustrated complicated process in vivo of Breviscapine. Intestinal absorption dynamics provided a method for profound study on the process in vivo and new oral druges development.