| Viral pneumonia is a serious disease that threats human health. It is of serious symptom andhigh fatality rate, and lack of effective therapy. Traditional Chinese medicine (TCM) has itssuperiority in preventing and treating viral pneumonia. This thesis studied the TCM mechanism ofviral pneumonia and the mechanism of Gualougancao granules treating viral pneumonia. Thethesis includes theoretic research and experimental research.1. The theoretic research The results of the theoretic research are showed as following. Viral pneumonia belongs towind-warm and lung-heat disease or plague in TCM. Toxin, phlegm, heat, healthy energydeficiency, blood stasis are the important pathological roles of the disease. Healthy energydeficiency is the base of onset of the disease. Healthy energy struggling with vicious energy, andthe endogenous toxin constantly producing are the important pathological links of the disease.Phlegm, heat and toxin combining together, and occlusion of lung energy is the importantcharacter of the pathological mechanism of the disease. Blood stasis blocking up the lung meridianis the posterio pathological manifestation. The result of healthy energy struggling with viciousenergy is the key role of the prognosis and conversion of the disease. On the whole, phlegm, heatand toxin combining together in the lung, and healthy energy deficiency are the importantpathological status of the disease. Thus the therapy of clearing heat, resolving phlegm, detoxicationand improving healthy energy is the important treatment of the disease.2. The experimental researchObjective The pharmacodynamics studies are to confirm the effectiveness of GLGC (therapy of clearingheat, resolving phlegm, detoxication and improving healthy energy) on influenza viral FM1infected mice. Pathomorphology studies, lipid peroxidation injuries studies, immune regulationstudies and cytokine studies are to explore the immune inflammatory mechanisms of GLGC oninfluenza viral FM1 infected mice.Methods In the pharmacodynamics studies, we observed the effect of GLGC on PSP excretory of miceairway, the effects of GLGC on the life protection, weights, lung exponent and blood agglutinationof FM1 infected mice. In the pathomorphology studies, we observed the effects of GLGC on the lung appearancechanges, pathological changes, and ultrastructure changes of FM1 infected mice, using naked eye,Abstract -7-light microscope, electron microscope. In addition, we used semi-quantitative analysis to compareall groups.In the lipid peroxidation injuries studies, we observed the effects of GLGC on the SOD andMDA lung content of FM1 infected mice, including different phases 1st , 3rd , 5th and 7th day.In the immune mechanism study, we observed the effects of GLGC on the spleen T,B-lymphocyte proliferation, and activity of NK cell of FM1 infected mice, using MTT method.In the cytokine studies, we observed inflammation correlated cytokines (TNF-α, IL-6, MCP-1,IFN-γ and IL-10) lung and serum content of FM1 infected mice, using ELISA method, includingdifferent phases 1st , 3rd , 5th and 7th day.ResultsThe pharmacodynamics studies showed that GLGC could improve PSP excretory of miceairway; compared with model group, the death rate, lung exponent, and blood agglutination ofGLGC group reduced obviously, the average life time and the weight of mice improved obviously.The pathomorphology studies showed that model group lung appearance changes wereconsolidation and hemorrhage; the pathological changes were serious interstitial pneumoniachanges, showing bronchial epithelium shedding, alveolar interval thickening, many mononuclearcells infiltrating the alveolar wall and bronchiolar wall, producing consolidated regions, serouseffusion in alveolar cavities, compensatory pulmonary edema; the ultrastructure changes showedthat the number of pulmonary interstitial cells improving, interstitial edema, m...
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