Via The Portal Vein Within The Liver Bone Marrow Mesenchymal Stem Cell Transplantation In Experimental Research

Objective:To observe whether bone marrow mesenchymal stem cells (BMSC) have the potential to differentiate into any hepatocyte-like phenotype after HGF treatment or co-cultured with injuried liver tissue in vitro. Materials and Methods:BMSC isolated from rat femurs were cultured and passaged 3-4 times in the presence of HGF. Cells were harvested on days 0, 10 and 20d, and subjected to examination of any hepatocyte's characteristics by RT-PCR, Western blot and immunocytochemistry. Results:Expression of albumin and α-fetoprotein (AFP) at both mRNA and protein levels were detectable on day20. By contrast, c-Met mRNA was significantly decreased in BMSC in the course of HGF induction. With the co-culture, the albumin, AFP and cytokeratin 8(CK8) mRNA were expressed in BMSC. Western blotting and ICC analysis indicate the expression of albumin and CK8 in BMSC. Conclusion:BMSC was shown to differentiate into hepatocyte-like phenotypes given the HGF induction or co-culture with liver tissue, as an alternative source for adult stem cell transplantation in liver repair.Objective:To identify any hepatocyte-like phenotype BMSC after intra-portal vein transplanted into dysfunctional livers. This study was also designed to evaluate the liver function and histopathology changes after intra-portal vein BMSC transplantation to liver damaged rats. Materials and Methods:GFP stabled transfectioned BMSC cross-sex were intraportal vein transplated into CCL< damaged or Common Bile Duct Ligation(CBDL) livers, GFP and Y chromosomes gene Sry in situ hybridisation were used to tracking the BMSC. And the hepatic specific antibodies fluorescence immuno-staining assay was used to determine the hepatocyte-specific phenotype in recipients' livers section. And serum liver function changes were examined after BMCS transplantation. Results:.In the normal recipients' liver section, 5% parenchyma cell GFP positive, and BDL liver section 16% positive. Double fluorescent immunohistochemistry showed the expression of albumin or CK8(red) in the GFP positive (green) cell cytoplasm. And after BMSC intra-portal vein transplantation, level of serum albumin in BDL 2w and CC14 8w rats receiving BMSC transplantation were significantly higher compared with those of control groups. Conclusions:There are more donor cells survival in injured liver than in the normal liver. Some subpopulation in the BMSC could experss hepatocyte-like phenotypes after transplanted into the injured liver indicating BMSC differentiation potential into the hepatocyte in Vivo. BMSC could engraft into liver tissue and have the ability to ameliorate the injurious effects of short time BDL and long time CC14.