| Objective To investigate the growth pattern and surface markers of Bone marrow mesenchyaml stem cells(MSCs) of cirrhosis patient ,And explore whether it can be induced into hepatocytes by Hepatocyte growrh factor(HGF)and Interleukin-6(IL-6) in vitro. Give new concept to the choice of "Seed Cells" for liver tissue engineer.Methods Bone marrow cells were collected from volunteers of cirrhosis patient aged from 30 to 40 in our department. MSCs were separated with Lymophocyte Separating Liquid (density 1.073g/L ficoll), biological features of cultures MSCs were observed under inversion microscope and their growrh curves were investigated, CD44 and CD45 was analyzed by flow cytometry. In this study MSCs passaged 3 were divided into 2 groups:group A wirh 20ng/ml HGF and 10ng/ml IL-6 in basical culture medium(L-DMEM with 10% FBS), group B just with basical culture medium respectively. The morphological changes of MSCs were observed by phase-contrast microscope and the expressions of alphafetoprotein(AFP), cell keratin 18(CK18) of each group were detected by immunohistochem technique on 0, 7, 14, 21, 28 days during differentiation.Results The latent phade of the primary culture cells was 0 to 9days, followed by 10 to 19 days of logarithmical proliferation, the plateau began at day 20 to 24. Subcultures showed faster growrh than primary cells, The latent phade was 4 to 24 hours, followed by 3 to 12 days of logarithmical proliferation,the plateau began at day 13 to 15. The MSCs expressed positive CD44 unanimously, but don't expressed CD45. Immunocytochemical analysis for AFP and CK18 showed positive staining reaction and negative staining resction in group B.Conclusion The biological features of MSCs of cirrhosis patient are as healthy as healthy human, and can be induced into hupatocyte lineage in the condition of HGF and IL-6 in vitro. It can be "Seed Cells" for liver tissue engineer and autologous MSCs transplantation .
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