Dyslipidemia Experimental Study Of The Compliance Impact Of The Sclera Of C57bl Mice

OBJECTIVE To examine the relation among scleral compliance, Phlegm-blood stasis, and age-related macular degeneration (AMD), the polarized microscope and transparent electronic microscope were used combined with picrosirius and oil-red "O" dyed methods. The changes of scleral collagen were studied. The affect of laboratoral dyslipidemia in scleral compliance of C57BL mice was observed.METHODS Three-month-old C57BL mice were chosen, they were divided into three groups: group 1, 12 mice, 3-month-old with normal feeding for one week and killed; group 2, 12 mice, 22 weeks with normal feeding up to 8-month-old and killed; and group 3, 18 mice with cholesterol-rich diet for 4 weeks. After 4 weeks take 6 mice from group 3 and killed for testing TC and LDL-C. The rest 12 mice will be fed with same food for 22 weeks and then killed. For three groups all test included the serum lipid levels , blood-rheology levels and MDA were determined, the eyes and aorta were ultrastructurally examined using picrosirius and oil-red "O" dyed methods to measure the changes of it. Histology study had been done with polarization microscope and transparent electronic microscope.RESULTS After 4 weeks, the 8 month cholesterol-rich feeding group showed increase of the TC and LDL and had big difference compared with 3-month normal feeding group(p<0.01), It confirmed the animal model of dyslipidemia .The serum lipid level: 8 month normal feeding group was higher than 3 month normal feeding group(p<0.05); 8 month cholesterol-rich feeding group was higher than 3 and 8 month normal feeding groups(p<0.01). Hemorrheology: 8 month normal feeding group was higher than 3 month normal feeding group(p<0.01); 8 month cholesterol-rich feeding group was...