Monkshood, Aconitum, Aconitum, Aconitum Leaf Time, Dose And Efficacy Study

Objective:To research correlation between decoction time(DT), administration dosage(AD) and the pharmic efficacy of lateral root of aconite(LRA), Sichuan aconite root(SAR), kusnezoff monkshood(KM) and kusmezoff monkshood leaf(KML) ; confirm the best DT and AD of the pharmic efficacy, and probe into the functional mechanism of LRA resisting Adjuvant Arthritis(AA).Methods : According to the efficacy of LRA, SAR, KM and KML, we chose several animal models, such as a heart failure model, a constipation model due to insufficiency of yang, kidney Yang deficient models, ache models, inflammation models and pyrexia models, and confirmed the best DT and AD of different medications, by Uniform Design(UD) and Regression Analysis. By a protein chip, we studied the action of LRA on AA rats' cytokines --BL-1 α , BL-1 β , IL-2, IL-4, IL-6L, IL-10, γ-IFN, GM-CSF, TNF-α .Results:1. The LRA (including the CLRA, the WPLRA and the BPLRA) could increase the heart rate(HR) of heart-Yang deficienct model rats obviously, improve +dp/dt_max of left ventricle, and reduce -dp/dt_max of rats' left ventricle; improve kidney Yang deficiency rats' general state obviously, raise their body temperature, resume their rhythms of the section day and night, remarkably extend their swimming depleted time in low temperature; obviously ameliorate Yang deficiency and astriction mice' s symptoms. The LRA could shorten the defecation latent period, promote defecation amount, and improve peristalsis rate of intestines and stomach in evidence. Obviously suppressed the mice' s auricle swelling caused by xylene; opposed rats' feet swelling caused by egg white notably; suppressed rats' granuloma hyperplasia caused by croton oil and reduced extravasate. The LRA reduced mice' s spraining body number of times caused by acetic acid obviously, lengthened their latent period of spraining body notably; evidently extended their latent period of stimulation by hot board and improved pain threshold value. The best DT and AD were 6 hours and 12g/kg respectively for the LRA to warm theheart-Yang, the kidney Yang, and the spleen Yang and smooth defecation. The best DT and AD were 0.25 hour and 12g/kg respectively for the LRA to resist inflammation and relieve pain.2. The SAR (including the CSAR and the PSAR) could suppress mice's auricle swelling caused by xylene obviously; alleviate rats' feet swelling caused by egg white notably; suppress rats' granuloma hyperplasia caused by croton oil and reduce extravasate. The SAR reduced mice's spraining body number of times caused by acetic acid obviously, prolonged their latent period of spraining body notably; evidently extended their latent period of stimulation by hot board and improved their pain threshold value. The best DT and AD were 6 hours and 2. 4g/kg respectively for the SAR to resist inflammation and relieve pain.3. The KM (including the CKM and the PKM) could suppress mice's auricle swelling caused by xylene obviously; alleviate rats' foot swelling caused by egg white notably; suppress rats' granuloma hyperplasia caused by croton oil, reduce extravasate. The KM reduced mice' s spraining body number of times caused by acetic acid obviously, prolonged the latent period of their spraining body notably; evidently extended latent period of stimulation by hot board and improved their pain threshold value. The best DT and AD were 6 hours and 2. 4g/kg respectively for the KM to resist inflammation, prolong the latent period of spraining body, and improve the pain threshold value by hot board .4. The KML could reduce the body temperature of fever rats evidently. The best DT and AD are 6 hours and 0. 96g/kg respectively for the KML to allay fever.5. The LRA could remarkable resist AA rats' swollen in their sole , and decrease the level of BL-1 a ? BL-1 P , BL-2, BLHL BL-6, BL-10. IFN- Y ? GM-CSF, TNF- a .Conclusions:1. LRA has the efficacy of remarkable warming Yang, evidently resisting inflammation and relieving pain. The best DT and AD are 0.25 hour and 12g/kg respectively for LRA to resist inflammation and relieve pain. The best DT and...