Ginkgo Biloba Extract Treatment In Rats Of The Mechanism Of Liver Fibrosis Research

Hepatic fibrosis is predominantly characterized by excessive accumulation of extracellular matrix (ECM) components in the liver, caused by an imbalance between synthesis and degradation of ECM. Up to now, no effective treatment of hepatic fibrosis is available for clinical use, in particular, no method for enhancing; degradation and resolution of these deposited ECM. and reversing hepatic fibrosis. Ginkgo Biloba extract(EGB) is an extract from green leaves of the Ginkgo biloba tree. EGB has been shown to have a SOD-like activity and hydroxyl radical scavening activity. Accordingly, we guess EGB can inhibit oxidative stress and hasten recovery of hepatic fibrosis.So far, there is no systemic research in treating hepatic fibrosis by Ginkgo biloba extract(EGB). Therefore, we use EGB treating CCl₄-induced hepatic fibrosis, and investigate the effect of general state of health, indication of liver function and histopathology on hepatic fibrosis's rat. Also ,we explore the effect of the oxidative indication such as MDA, SOD and GSH-Px, the express of TGF-β₁ and Activin A , the activation of HSC, the apoptosis of liver cell, and the gene express of MMP-1 and TIMP-1. From the experiment, we will investigate the therapia effect of EGB on fibrosis's rat, and explore the treated mechanism from oxidative stress, molecular level, cellular level and degradation of ECM by the way. The experiment was divided into six part.Part 1: The effect of Ginkgo biloba extract on general state of health and indication of liver function in hepatic fibrosis's rat.Objective: To investigate the change of general state of health and indication of liver in the course of treating hepatic fibrosis's rat. Methods: The study was conducted in 48 adult male SD rats randomized into 4 groups: normal group, model group, treatment group and colchicin group. Except the rats in the normal group, others were inflicted with liver fibrosis by administration of CCL₄ for 8 weeks. Then, the rats of normal group and model group were sacrificed. Blood samples were collected for determination of ALT. AST. AKP and ALB. Those rats in the treatment...