Irritable Bowel Syndrome Slow Acute Joint Stress Rat Model Of Distal Colon Sympathetic Signal Intensity Changes

The reevaluation of the animal model of irritable bowel syndrome which combined chronic unpredicted mild stress with acute restrain stressBackground and aims: Irritable Bowel Syndrome (IBS) is a common functional bowel disease. Its etiology and pathophysiology are incompletely understood. Concurrent psychiatric morbidity is prevalent in IBS patients. Psychological stress is known to evoke or exaggerate its symptoms of IBS. The disorder of colonic motility, sensation, mucosal secretion and epithelial barrier function are main pathophysiologic manifestation of IBS. To establish an animal model is necessary to test pathophysiologic hypotheses of IBS. Based on the theory of brain-gut interaction, the animal model of IBS, which was treated by stimulation of chronic unpredicted mild stress with acute restrain stress, has been established in our previously study to mimic the manifestations of colonic motility, visceral sensation and behaviour in IBS. In the present study the animal model will be reevaluated in the above aspects and evaluated in colonic mucosa secretion and barrier function.Methods: Health male adult wistar rats were selected and divided into four groups: control, acute stress (restrain for 1 hour), chronic stress (chronic unpredicted mild stress for 21 days) and chroic stress combined with acute stress. We employed the technique of short circuit-current to study the effect of stress on the isolated mucosa secretion and barrier function in rat distal colon. We observed the consumption of sucrose solutions, the fecal pellets, the numbers of abdominal muscle contraction by colorectal...