Changes And Significance Of 5-hydroxytryptamine And Serotonin Reuptake Transporter In The Colon Mucosa Of Mice With Colitis Induced By Dextran Sodium Sulfate

Background: The etiology and pathogenesis of ulcerative colitis remain unknown. It is very important to develop a proper animal model for understanding the pathology of UC.Aims: we developed the acute mice dextran sodium sulfate ulcerative colitis and wanted to know the proper concentration and time of dextran sodium sulfate in inducing acute ulcerative colitis.Methods:Acute colitis was induced by the administration of 3%,5%,8% DSS in C57BL/6 mice, In control,mice drank distilled water. The colonic injury and inflammation were assessed by disease active index (DAI) and histological index (HI) to evaluate the model.Results: A linear increase in DAI was noted with increasing concentrations (1.50±0.53,7.71±1.38,9.14±1.03 and 9.71±0.61,respectively), and depended on the DSS concentrations( p<0.05 comparing all groups); A linear increase in HI of the distal colon (0,5.19±1.06,6.38±0.91 and 7.75±0.46, respectively) and proximal colon (0,3.50±1.07,5.12±1.55 and 6.62±1.41, respectively ) was noted with increasing concentrations, and depended on the DSS concentration( p<0.05 comparing all groups) ; The mortality increased with increasing (0,3%,5%,8%) DSS concentrations (0,7.10%,28.60%,57.10%,respectively).Conclusions: DSS-induced acute mucosal injury was dependent on DSS concentration. An ideal acute UC model might be developed by which C57BL/6 mice drank 3% DSS (molecular weight was 36,000～44,000) for five days .