Hypoxia On Human Embryonic Lung Fibroblast Cell Proliferation, I, Iii Procollagen Gene Expression And Its Mechanism Discussed

The proliferation and procollagen gene expression of fibroblast, the most numerous intrinsic cell in the connective tissue, affect the outcome of wound healing. Hypoxia is an important environmental factor that plays an important role in the fibroblast proliferation and procollagen gene expression during the wound healing process. The goal.of this study is to gain insights into the underlying mechanisms of inflammation, anti-inflammation and wound healing. We observed the effect of hypoxia on the proliferation and α₁ (I,III) procollagen gene expression of cultured human fetal lung fibroblast and examined the probable mechanisms in these events.1. Culture and characterization of the human fetal lung fibroblast, establishment of the hypoxic fibroblast model and the assessment of the hypoxia-induced cell damage.Cultured human fetal lung fibroblast was prepared with tissue piece method and characterized with light microscopy, transmission electron microscopy, seeding viability assay and immunocytochemical assay. We prepared the hypoxic (2% oxygen) human fetal lung fibroblast model and assessed the hypoxia-induced cell damage with light microscopy, transmission electron microscopy, seeding viability assay and DNA integrity assay. The results showed no significant difference between the hypoxic fibroblast and that under the normal oxygen tension. It could be concluded that the viability of cultured human fetal lung fibroblast did not change under the condition of hypoxia (2% oxygen).