Since The Body Material Of Non-neural Tissue Transplantation To Repair Facial Nerve Defect Animal Experiments

Facial nerve or other peripheral nerve injuries that result in a nerve gap remain challenging surgical problems. Although autogenous nerve grafting (ANG) is still considered the current reliable method for repairing nerve defects, it has the disadvantage of sacrificing one or more nerves, resulting in dysfunction at donor-site, and of sometimes being unavailable. It has long been researched in alternating autogenous nerve grafts with other materials. In the present experimental research in animals, the feasibility of substituting autologous non-neural tissues graft for autogenous nerve graft for the repair of facial nerve defects was investigated.Rabbit facial nerve defects were repaired with combined inside-out vein and skeletal muscle grafts, to which no other intervention was added. The efficacy and pathaphysiological mechanism of this method were observed respectively in two portions: (1) Evaluation of combined inside-out vein and skeletal muscle grafting for the repair of facial nerve defects with different distances: Twenty healthy male New Zealand rabbits weighing range 2~2.5kg were used in this experiment and were divided randomly into four groups of five rabbits each. In all groups, the superior buccal branch of facial nerve on right side was used as the experimental subject. In two of the four groups, 10mm nerve gap was created (short nerve defect groups); in the others, the gap was 20mm long (long nerve defect groups). In one of the two short nerve defect groups, the nerve defects were repaired by combined inside-out vein and skeletal muscle grafts (short combined inside-out vein and skeletal muscle grafting group), which was controlled to traditional autogenous nerve grafts in the other (autogenous nerve grafting group). In one of the two long nerve defect groups, the nerve defects were repaired with combined inside-out vein and skeletal muscle grafts (long combined inside-out vein and skeletal muscle grafting group), which was compared with standard vein and skeletal muscle grafts in the other (combined standard vein and skeletal muscle grafting group). At six...